Function Of PKC-NF-κ B Signal Transduction Pathway In The Proliferation Of Ovarian Cancer

PurposeProtein kinase C (PKC) is a cytoplasmic serine / threonine protein kinase. It can be activated by phosphorylation of transcription factor AP-1 and NF-κB, and so on. Then it will cause the promotion of target gene transcription and cell proliferation and hypertrophy. Nuclear factor-κB (NF-κB) is a key transcription factor involved in cytokines, adhesion molecules and vascular activity factor adjustment. It is now widely recognized that NF-κB related to the formation, growth, treatment and prognosis of malignant tumors. More and more researches show that PKC-NF-κB signal transduction is important in cell growth, metabolism and apoptosis. It is closely related to malignant transformation. We try to explore the effect of PKC-NF-κB signaling pathway on ovarian carcinoma cell and its mechanism. Our aim is to investigate in order to find the new methods for the tumor treatment.MethodsSelected wax lumps included 49 ovarian carcinoma, 10 benign epithelial ovarian and 10 ovarian normal tissue to check the expression of PKCαand the NF-κB by the immunity organization S-P method.Results1. The expression ratios of PKCαin benign epithelial ovarian and in ovarian carcinoma were 20.00% and 59.18% respectively, having significant difference (p<0.05). The NF-κB expression level positively correlated with pathologic grade (p<0.05),while no significant difference was observed in age, clinical stages and the histopathology. 2. The expression ratios of NF-κB in benign epithelial ovarian and in ovarian carcinoma were 10.00% and 69.39% respectively, having significant difference (p<0.05).The NF-κB expression level positively correlated with pathologic grade (p<0.05),while no significant difference was observed in age, clinical stages and histopathology.3. There were statistically significant correlation among PKCαand NF-κB.Conclusion1. There was an over expression of PKC and NF-κB in ovarian cancer. Their expression level positively correlated with pathologic grade.2. There was a significant correlation between the expression of PKCαand NF-κB.