Research On Antihypertensive Effect And Mechanism Of The Extract Of Ocimum Basilicum L (OBL) In Renal Hypertensive Rats

Object: To investigate the effect of the extraction of Ocimum basilicum L (OBL) on renal hypertensive rats and investigate its antihypertensive mechanism. Methods: SD rats received food and water adlibitum in 1 week of acclimation. After that baseline blood pressure (sistolic and diastolic blood pressure) was measured by noninvasive tail cuff photosensor under aseptic conditions, thirty general anesthesia rats were given a modifided 2kidney- 1clip(2K1C) operation while six rats were sham-operated (the animals received identical treatment without left renal artery ligation). Four weeks later, tweniyfour hypertensive rats were randomly allocated to four groups such as OBL (higher, 400mg/kg), OBL(midel, 200mg/kg), OBL(lower, 100mg/kg), captopril (cap, 30ml/kg) and model group after allocation, the treatment period was 4 weeks. The blood pressure was measured weekly. In the 4th week, blood urea nitrogen(Bun) and serum creatinine (Scr) were measured too. At sacrifice, blood was harvested to measure the levels of angiotensinⅡ(AngⅡ), superoxide dismutase (SOD), malonaldehyde(MDA) and endotnelin (ET) at the end of the 4th week. Kidneys and hearts were collected to observe the patho1ogical changes of arteriole too. Results: Four weeks after 2K1C operation, the blood pressure of the 2kidney- 1clip operation group increased significantly(138±2.04 mmHg vs 201±3.69 mmHg of sham group, P<0.05). and the levels of angiotensinⅡ(AngⅡ), malonaldehyde (MDA), endotnelin (ET), blood urea nitrogen (Bun) and serum creatinine (Scr) was slightly increased in plasma. Superoxide dismutase (SOD) was decreased significantly. Treated with captopril and Ocimum basilicum L (OBL), the blood pressure(sistolic blood pressure and diastolic blood pressure) decreased significantly and continually (P<0.05). During the course of administration, the levels of angiotensinⅡ(AngⅡ), malonaldehyde (MDA) and endotnelin (ET), blood urea nitrogen (Bun) and serum creatinine(Scr) was slightly decreased in plasma. mean while, superoxide dismutase (SOD) in plasma increased respeetively(P<0.05). In OBL groups, due to the short course of experiment, the pathological change of coronary artery and renal artery was not seen under the microscope. Conclusions: Ocimum basilicum L (OBL) can reduce the blood pressure of the renal hypertensive rat, Which is likely related to throu the antioxidate rection, to improve the blood vessel endothelium function and inhibited the material of vessel contraction release. It should be more than one antihypertensive target of Ocimum basilicum L (OBL).