The Relationship Between The Single Nucleotide Polymorphism Of The Fibrinolytic Gene And Ischemic Stroke In Chinese

Background and Purpose Atherosclerosis is one of the most important pathogenesis mechanisms in stroke. Traditional risk factors for stroke such as hypertension, obesity, cigarette smoking, age, diabetes and hyperhomocysteinemia may lead to atherosclerosis. But these factors could only explain about one third the development of stroke. The purpose of this study is to explore the association between TPA gene -7351 Polymorphism and Ischemic stoke in Chinese.Methods Samples of peripheral blood were collected from 140 patients with ICH diagnosed by CT or MRI from seven areas of Hu Bei province in China and 140 age, sex and geographically matched subjects as controls. Participants were evaluated for known cerebrovascular risk factors, and the TPA -7351C/T genotype was established by a allele-specific polymorphism chain reaction method. Logistic regression was used to determine the risk of lacunar and nonlacunar ischemic stroke associated with the TPA -7351C/T polymorphism. Results (1) The prevalence of the TPA -7351CC, CT, and TT genotypes were 45.1%, 44.4% and 10.5% for controls and 43.6%, 26.4%, and 30% for stroke patients, respectively. (2) After adjustment for known cerebrovascular risk factors, the TT genotype was significantly associated with ischemic stroke (OR:1.446;95% CI:1.273-6.388) (3) Static life-fashion is an important risk factor of ischemic stroke. Taking exercises was identified to can decrease the risk of Ischemic stoke.Conclusions: the TPA -7351 polymorphism is an independent risk factors for ischemic stroke. The findings suggest that impaired fibrinolysis may play a role in the pathogenesis of ischemic stroke. Objective The tissue-type plasminogen activator (TPA)-7351C/T and the plasminogen activator inhibitor type 1 (PAI-1)675 4G/5G polymorphisms influence transcriptional activity. Both variants have been associated with myocardial infarction, with increased risk for the T and 4G allele, respectively. In this study we investigated the possible association between these polymorphisms and ischemic stroke.Methods Stroke subtype was determined using Classification of cerebrovascular diseas (1995). Samples of peripheral blood were collected from 121 patients with ICH diagnosed by CT or MRI from Hu Bei province in China and 141age, sex and geographically matched subjects as controls. Participants were evaluated for known cerebrovascular risk factors, and the TPA -7351C/T genotype and PAI-1 675 4G/5G genotype were established by allele-specific polymorphism chain reaction methods. Logistic regression was used to determine the risk of ischemic stroke associated with the TPA -7351C/T polymorphism and. PAI-1 -675 4G/5G polymorphism, and results were considered statistically significant at P<0.05 using a 2-tailed test.Results. The multivariate-adjusted odds ratio for overall ischemic stroke was 0.264(0.109-0.668) for the PAI-1 4G allele carriers, P value<0.001; 0.703(0.459-1.078 ) for subjects homozygous for the PAI-1 5G allele, P value<0.05. When genotypes were combined, no protective effect for the TPA CC/PAI-1 4G4G genotype combination was observed (odds ratio 0.972, 0.235 to 4.027; P>0.05).Conclusions The PAI-1 - 675 4G/4G genotype nor the PAI-1 - 675 5G/5G genotype showed association with ischemic stroke. For the TPA CC/PAI-1 4G4G genotype combination, no protective effect was observed. Collectively, these results are consistent with a more complex role for TPA and PAI-1 in the brain as compared with the heart.