The Isolation And Vitro Antitumor Activity Of Compounds From Juglans Mandshurica Maxim

Malignant tumor is a clinical common disease, threating the person's physical and mental health severely. In the world, the death rate of the tumor is the first or the second in all of the death causes and that has been the second in China. Tumor is a refractoriness disease because of the multiplicity of etiopathogenisis. Drug treatment, operation cure and radiation therapy are the important methods of cancer treatment. Chemotherapy is circumscribed in clinical application because the chem drug has severe side effect. Traditional Chinese drug is a traditional method of cancer therapy and in the advantage of its less side effect, it is becoming the considerable treatment in tumor combined therapy .Juglans mandshurica maxim belongs to Juglandaceae. It is distributed over north-east, north of China, HeNan province and ShanXi province in China. Its root, stem-bark, leaves and exocarp (qinglongyi) have pharmacologic action including antibiosis, anti-virus, analgesia, anti-oxydation, anti-tumor and et al. Especially anti-tumor effect is paied closed attention by much researcher, who concentrate more investigation on anti-tumor effects of Leaves and qinglongyi. The results show that the extracts of leaves and qinglongyi can inhibit multitude kinds of tumor cells proliferation, induce the apoptosis of tumor cells, make telomerase activity down, block cell cycle, increase immune function of mice carrying tumor, influence the tumor cell membrane fluidity and erythrocyte membrane fluidity, promote the expression of TNFα-mRNA in peritoneal macrophage of mice and so on. Qinglongyi has been used for cancer treatment in clinic, which gain satisfied effects.Although the chemical structure and pharmacologic action of Juglans mandshurica maxim have been rescearch profoundly, there is little rescearch on its anti-tumor activity which is used to cure a lot of kinds of tumor in the folk. We used the method of vitroantineoplasmic activity to trail the component of anti-tumor. We carried out some research on the isolation of Juglans mandshurica maxim and the antieoplasmic activity of the components in the Juglans mandshurica maxim.Our research included several aspects. First, we reviewed chemical sonstituent and biological activities of Juglans mandshurica maxim. Second, though using solvent extraction, SG column chromatography and ODS column chromatography were compunds isolated from Juglans mandshurica maxim. The structure of these compounds are elucidated by physico-chmical sonstants and spectroscopic analysis. It is: Fourth, through MTT and XTT, we discovered that the play a definitive role on anti-tumor activity.