| Ischemic heart disease (IHD) is caused by coronal blood circulation changes and induces the imbalance of myocardial supply and demand. IHD includes myocardial ischemia which arises from functional and organic alteration of the coronal arteries, which in turn, comes about due to acute and chronic diminished blood supply. IHD includes myocardial ischemia which arises from functional and organic alteration of the coronal arteries, which in turn, comes about acute and chronic diminished blood supply. Its most common pathogenisis is the construction and obstruction of the coronary arteries resulting from atherosclerosis. Therefore, IHD is also called atheros clerotic heart disease. Heart is the larges oxygen-needing organ in body. Heart needs numerous oxygen supplies in time. Blood circulation ensures myocardium acquire sufficient oxygen and nutrition and drain metabolize outcome. The decrease of blood irrigation caused by any reason can bring about ischemia injury to cells. Resuming blood irrigation of tissue as soon as possible is the basic measure to decrease ischemia injury. In recent years, along with the rapid development of iatrotechnics, the ischemia organ and tissue can require blood irrigation again more quickly, which can decrease cellular injury and increase curative effect. However, we found by animal experiment and clinic observation that part of animals or patients will aggravate the obstacles of function and metabolize and the destruction of cell structure. The phenomenon that blood irrigation can aggravate ischemia injury is called ischemia-reperfusion injury. It is a important task to be resolved in preventing and treating IHD that not only resume the blood irrigation but also prevent or mitigate ischemia reperfusion injury. About the mechanism of IRI there are mainly falling several theorys: 1.oxygen free radical damage 2.calcium overload 3.disturbance energy metabolism of cardiac muscle fiber 4.granulocyte infiltration 5. cellular adhesion molecule 6. complement 7. apoptosis. And to serve asa go between those theorys is the importance of free radicals in the prosess of IRI. In the nowdays, cardiovascular diseases are contracting more and more frequently, and myocardium is in the danger of IRI. It has important theory basis and practice value to develop drugs which can prevent the ischemia reperfusion injury myocardium cells.So in our experiment, we designed and made a model in which the myocardium of rats were injured by ischemia reperfusion. we observed the effect of DhHP-6 on experimental anoxemia of myocardium douche injury. contents of SOD MDA in cardiac muscle tissue and LDH AST and CK in blood serum can be measured. Survey histopathological change of rat cardiac muscle.The ischemia-reperfusion-injury model of myocardium in rats was successfully improved, and electrocardiogram was choosed to ensure that prosseses of ischemia reperfusion had really happened. The rat myocardial ischemia model is resulted from injection of DhHP-6, could decrease ST length and decrease the myocardial ischemia coverage.LDH AST and CK release, decrease SOD activity, and MDA generation after ischemia reperfusion,inhibite LDH AST CK released,and improve SOD activity. MDA contents of groups are reduced significantly.Based on the above experiments, it has been proved that increased the ability of removing free radicals, decreased lipid peroxidatic, protect both integrity and stability of cellular membrane, and then protected the cardiac muscle cell from hypoxia damage.
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