| Background and Objective Neuroblastoma is one of the most common malignant tumours in children,and there are no effective therapeutic approaches for the diseases in spite of advances in surgery, chemotherapy, and radiotherapy. For this reason, it is important to research and develop new methods for treatment of neuroblastoma . Among the new strategies for cancer therapy, differentiation therapy has been widely studied in recent decades. Our previous studies have showed that nordihydroguaiaretic acid(NDGA) could inhibite growth of glioma in vivo and in vitro, but its effect on neuroblastoma is unknown. In this study, the effect of lipoxygenase inhibitior, nordihydroguaiaretic acid(NDGA) on the growth of human neuroblastoma cell line SK-N-SH cells was investigated , we hope we would provide a few certain proofs for clinic therapy with lipoxygenase inhibitior through evaluating the growth-inhibiting and apoptosis-inducing effects of NDGA on human neuroblastoma cell line SK-N-SH cells in vivo.Methods SK-N-SH cells were cultured in vitro and seeded into culture plates. At the state of culturing, observe the morphology of cells in culture flask by invert microscope every day. Following exposure to NDGA at different concentrations in different times, then SK-N-SH cells were harvested and investigated. Cells growth inhibition was evaluated by MTT assay, cell quantification and colony-forming test. HE staining ,FCM and TUNEL staining were used for the analysis of apoptotic.Results (1)The growth inhibition of SK-N-SH cells line induced by NDGA was enhanced in a time-dependent and dose-dependent manner and the highest inhibitory rate was 63.6%. The colony-forming rates were 74.8% and 23.2% respectively(P<0.05).(2)The morphological changes of apoptosis were observed in SK-N-SH cells after treated with NDGA for 72h, including cell shrinkage , chromatin condensation, chromatin crescent, and nucleus fragmentation.(3) TUNEL staining and FCM assay showed that apoptotic rate of SK-N-SH cells increased in a dose and time dependent manner after exposure to NDGA (5μmol/l and 100μmol/l) for 48h and 72h.But the cell cycle of SK-N-SH cells was not affected.Conelusions (1) Lipoxygenase inhibitior NDGA can significantly inhibit the proliferation of f SK-N-SH cells in vitro in a dose-dependent and time-dependent manner. (2) NDGA could induce apoptosis in neuroblastoma cell line SK-N-SH in vitro in a dose-dependent and time-dependent manner. |
