Relationship Between The Single Nucleotide Polymorphisms (SNPs) Of CTLA-4 Gene And Hepatitis B

Background The infection of hepatitis B virus becomes one of the most severe public health problems all over the world.Its mechanism is very complex.At present,most people think that it maybe be associated with many factors,such as virus,the environment and host immune function.Some research show,the virus infection situation partly depends on the host susceptibility.By now,more and more people focus on host susceptibility,the research about this will be a hot spot.Objective To investigate the single nucleotide polymorphisms(SNPs) of cytotoxic T lymphocyte antigen-4(CTLA-4) gene,study the relationship between the SNPs and the turnover of the chronic hepatitis B infection,research the mechanism of the hepatitis B.Methods The restriction fragment length polymorphism(RFLP)-polymerase chain reaction(PCR) was used to analyze the CTLA-4 exon 1+49,promoter -1661,-1722 polymorphism in 190 chronic hepatitis B patients and 93 healthy controls,also study the relationship between the SNPs and turnover of the hepatitis virus B infection.Results The frequency of genotype at the exon 1+49 site,promoter -1661,-1722 site were statistically different in healthy controls and in chronic hepatitis B patients(P＜0.05).The frequency of A allele at the exonl +49 site,promoter -1661 site were statistically higher in chronic hepatitis B patients than in healthy controls.(0.439 vs 0.323,P＜0.01;0.839 vs 0.742,P＜0.01).The frequency of C allele at the promoter -1722 site was statistically lower in the chronic hepatitis B patients than in healthy controls.(0.321 vs 0.425,p＜0.05). Conclusions The G allele of CTLA-4 exon 1+49 and promoter -1661 site polymorphism,the C allele of promoter -1722 site polymorphism show highly significant association with chronic hepatitis B patients.