Studies On Sensing Detection Of Lactose And In Situ FTIR Analysis Of Cancer Cell

Since 1980s, biotechnology, new materials and information technology have been regarded as the symbols of technical innovation, which are important to the areas of population, environment, food, energy, health and so on. This dissertation focuses on the areas of life science and materials. We developed a magnetoealstic biosensor to determine the lactose in milk sample, studied the application of in situ attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy in analysis of cancer cells. Finally, we investigated the graphitization of carbon fibers.(1) Analysis of lactose in milk samples by wireless sensing: We developed a wireless biosensor for analysis of lactose in milk by co-immobilizingβ-galactosidase (GLA), glucose oxidase (GOD) and catalase onto a magnetoelastic ribbon-like sensor which was pre-coated with a layer of pH-sensitive polymer. The lactose can be hydrolyzed to produce acid finally by the multi-enzyme, resulting in the pH-responsive polymer shrinking, and consequently the resonance frequency (?r) of the sensor increasing. The shifts in frequency were linearly proportional to the concentration of lactose from 2 mmol/L to 16 mmol/L with a detection limit of 0.72 mmol/L. The sensor was applied to the milk samples, and the results were the same as that of spectroscopic analysis.(2) In situ evaluation of breast cancer cell growth with ATR-FTIR Spectroscopy: Base on the powerful ability of Fourier-transform infrared (FTIR) spectroscopy to elucidate the structure of biological samples. We introduced ATR-FTIR to in situ evaluate the growth of human breast cancer cell, and elucidated the distinguishing phase of the cell cycle with 3D ATR-FTIR spectroscopy. The different spectroscopy between cancer cell and normal cell offered information to the cancer screening. This study not only provides a time sketch of cancer events but also shows the potential to the mechanism of the anti-cancer drugs acting on the cells.(3) The investigation of effect on the graphitization of carbon fibers with electroless deposition of Ni-P catalyst under a magnetic filed: In the process of electroless deposition of Ni, a magnetic filed was added to obtain a homogenous, smooth and orientated coating. SEM images determined the surface of two different coatings. A conclusion that the graphitization is independent on the degree of smooth of coating surfaces was achieved by XRD data.