| As people have realized the toxic effects of chemical medicine and have improved their health consumption conceptions, the use and requirements of natural medicines have been expanded constantly. More and more people have emphasized the characters and advantages of Chinese herds. How to completely extract active components and improve the utilization and clinical effects of traditional Chinese herbs, have become the main subject and task of traditional Chinese herbs in its modernization. The noval types of sample pretreatment techniques (microwave-assisted extraction approaches) have been used for extraction and isolation of polyphenolic compounds, such as chlorogenic acid, caffeic acid, syringin, et al, from Eucommia ulmodies Oliv. E.ulmodies is one of the precious Traditional Chinese Medicine which distributes in the west of Hunan province. In this work, the parameters which influence the extraction efficiencies in each technique had been investigated. Additional, the quality control standard of Eucommia ulmodies Oliv was established, which was used to control the quality of E.ulmodies. Then the research about trace elements and antibiotic functions had been done, which can provide reference informations for the rational use of E.ulmodies. The concrete contents are as follows:1. The research of extraction of Chlorogenic Acid from E. ulmodiesThe optimum technics of extracting chlorogenic acid from E.ulmodies was studied. During the extracting process, extracting solvents, methods and conditions were studied. The result showed that: Microwave-assisted extraction was fast and efficient. The optimum conditions were: the volume fraction of ethanol, 0.6; the pressure, 2atm; time, 2min. The material/solution ratio was 1:50. Under the same HPLC condition, we detected the content of chlorogenic acid in leaves, unroasted bark, roasted bark and Du Zhong Ping Ya Pian. The content of chlorogenic acid in leaves was higher than that in bark. Meanwhile the leaves of E.ulmodies were easy to get, it can provide abundance chlorogenic acid resource. Microwave-assisted extraction was a kind of newfashioned, high efficient, convenience and fast extraction mean, meanwhile the HPLC method had good precision, repeatability. The result was dependable. It can be adopt to determinate the content and control the quality of material and products that contain chlorogenic acid. 2. Selection and fingerprints of the control substances for plant drug Eucommia ulmodies Oliv by HPLC and LC-MSThe selection of the control substances and their fingerprints of this plant medicine were investigated by using HPLC and LC-MS. The gradient elution mode was applied in chromatographic separation, and the data were analyzed by"Computer Aided Similarity Evaluation"software to compare the similarity of the E. ulmodies from different habitats. Low similarity was found in the samples from nonadjacent provinces, while high similarity was obtained in those from the adjacent provinces. The LC-MS fingerprints of E. ulmodies showed the main active constituents and could be used for its original identification and quality evaluation.3. Determination of elements in the E.ulmodiesThe E.ulmodies is a miraculous tree. Its leaves bark and flowers contain trace elements in human. The elements of the E.ulmodies were determined by FAAS. The results showed that: There were abundance elements, such as Mg Cu Fe, et al. The contents of them were higher in leaves than in bark, and they played a very important part in the metabolish of the flesh. While the contents of the element, such as Pb Cr, were very low. So E.ulmodies is a very safe Chinese traditional medicine. The experiment results provided the scientific basis for further research. Meanwhile by comparing different methods, the microwave digestion had the merits of efficiency, rapidity, less pollution and convenience. It is the best sample preparation method for determination plant's trace elements contents.4. Study on antibiotic ability of leaves extracts of E.ulmodiesChlorogenic acid was extracted from E.ulmodies by Microwave-Extraction method. The solvent was a mixed solution of ethanol and water. The antibiotic ability of chlorogenic acid under optimum extraction condition was studied. The result showed that, the extract had the antibiotic ability towards Escherichia coli, and the MIC was 100 mg/mL.
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