The Expression And Significance Of Endocrine Gland-derived Vascular Endothelial Growth Factor In Polycystic Ovaries

ObjectivesPolycystic Ovary Syndrome(PCOS)is the most common cause of infertility reason,there are about 75%aovulation infertility for PCOS.Hyperplasia and hypervascularity of the ovarian theca interna and stroma are prominent features of PCOS.Angiogenesis and its correlation factors are associated with the progression of PCOS.Endocrine gland-derived vascular endothelial growth factor(EG-VEGF)has been recentlAna 1 aviadenovirusidentified as an endothelial cell mitogen with selectivity for the endothelium of steroidogenic glands.EG-VEGF is expressed in the organs secreting steroid hormone such as ovaries and adrenal gland and isn't expressed in other organs.Vascular endothelial growth factor(VEGF)is a key regulator of normal and abnormal proliferation of blood vessels without selectivity for vascular endothelial cell.The internal study of EG-VEGF and VEGF in PCOS is still empty worldwide and the overseas study is also seldom.We plan to study the expression of EG-VEGF in pathological changes of the Polycystic ovaries via immunohistochemistry and to compare with VEGF,in order to explore the role EG-VEGF play in development and progression of PCOS and to establish rationale for gene therapy of PCOS.Materials and MethodsSample:63 paraffin samples pathological changes of Polycystic ovaries tissue were collected from the Shengjing Hospital of China Medical University,September 1995 to February 2007.And additional normal ovaries tissue 20 cases for comparision, including follicular phase 10 cases and luteal phase 10 cases.Reagent:polyclonal rabbit anti-human VEGF antibody purchased from Boshide biology Ltd.Corp.Polyclonal goat anti-human EG-VEGF antibody purchased from Santa Cruz Biotechnology Ltd.Corp.Streptavidin peroxidase(SP)reagent cases purchased from Zhongshanjinqiao Beijing biology Ltd.Corp.Diaminobenzidine(DAB) purchased from FuzhouMaixin biology Ltd.Self confected antigen repaired buffered liquid and phosphate buffered solution(PBS).Experimental Methods:The experiment was conducted by immunohistochemistry according to instructions of the reagent cases.The diluted concentration of the first antibody VEGF is 1:75,and that of EG-VEGF is 1:50.This study used pre-existing positive sections of placenta as comparison samples,and used phosphate buffered saline (PBS)instead of the first antibody as negative comparison samples.Every sample is stained with Hematoxylin.Results Determination:Brown-yellow particles which distributed in the cytoplasm of polycystic ovaries of different patholigical changes mean positive expression of EG-VEGF and VEGF.Image Analysis:selected 6 complete and nonoverlapping fields of vision for each case,determined optical densities of immunoreaction positive particle of EG-VEGF and VEGF and regarded the average value of the 6 optical densities as the measure value for each case.Statistical Analysis:To adopt two-sample t-test,linear correlation analysis.A probability value＜0.05 was considered statistically significant.ResultsThe expression levels of EG-VEGF in the study group were significantly higher than those in the control group(P=0.001).The expression levels of VEGF in the study group were significantly higher than those in the control group(P=0.021).The expression levels of EG-VEGF in the study group were higher than that of VEGF(P=0.036),but there was no dependablity between the two factors(P=0.327).The expression levels of EG-VEGF in the control group were a little higher than that of VEGF,but there were no statistically significant(P=0.655),and there was no dependablity between the two factors(P=0.928).ConclusionOverexpression of EG-VEGF promote angiogenesis of Polycystic Ovary Syndrome and is associated with the progression of Polycystic Ovary Syndrome.