| PrefaceThe incidence of brain injury and accompanied mutilation and mortality rates have increased year by year with the development of the economy and transportation.The abnormal expression of cytokines in traumatic brain injury after drug treatment was investigated to illuminate the mechanism of cerebral injury,and thus to direct the clinical treatment,which produces the elevated recovery rate and decreased mortality and mutilation rates.The tumor necrosis factor-alpha belongs to pro-inflammatory cytokine,which increases obviously after brain injury and plays an important role in the secondary brain injury,the magnesia mixture and naloxone currently have certain protection for traumatic brain injury demonstrated in many researches,but the mechanism has not been illuminated completely.In this study,the animal model for brain injury by the Feeney method of free falling object was established,and given magnesium sulfate and naloxone.The changing in the concentration of TNF-αand brain edema in rats' brain tissue was observed to investigate their brain protective mechanism and the effect of combined medication.Material and MethodsTotal 60 Wister masculinity rats were included in this study,whose body mass were from 270g to 300g,and randomly divided into four groups:vulnerate control group,naloxone group,magnesium sulfate group and combined medication group. Each group was subdivided into three time points:6h,12h,24h after injury,and there were 5 rats in every time point.Experimental rats were anaesthetized through injecting 10%chloral hydrate(3mg/kg)into abdominal cavities,and a diameter of about 5mm bony window was drilled in every rats in about 2mm to the right of the median line and about 2mm to the post of sutura coronalis.The device of Feeney free falling body was used to injury the rats.After injury,every group was immediately given peritoneal injection naloxone(1mg/kg),12.5%magnesium sulfate(300mg/kg),12.5%magnesium sulfate(300mg/kg)+naloxone(1mg/kg),and equivalent normal sodium to the control group respectively.The rats were put into death at the time of 6h,12h,24h respectively after injury,and to get brain tissues.Partial brain tissues were immediately put into 10%formaldehyde and made into wax sample after fixing;and partial brain tissues were reserved in refrigerator with temperature70℃below zero.The moisture in the pallium at the edge of contusion foci of rats was calculated according to Elliotl formula in every therapeutic group and the control group,the contents of TNF-αwere determined through ELISA method,and the sample was obtained for pathologic examination.Statistical analysis:all measurement data were expressed as(?)±s,and statistical significance was evaluated by multiple comparison of test and ANOVA.A P value of 0.05 or less was considered to indicate statistical significance.All statistical processes were completed via SPSS(V13.0).ResultsUnder the macroscope,obvious inflammatory reaction,cellular edema and inflammatory cell infiltrating were found after cerebral injury.The moisture of brain tissues at 6h,12h,24h after brain injury in rats was compared,and the increasing trend was obvious(every two time points was compared in the three points,P＜0.05),and the moisture of brain tissue at 24h after injury was highest.Comparing the three therapeutic groups and control group,the moisture of brain tissues were all decreased (medication alone P＜0.05,combined medication P＜0.01),most obviously in combined group,and the combined group was more obvious different than medication alone(P＜0.05),these results indicated that during 24h after brain injury of rats,brain edema was increased trend,and combined magnesium sulfate with naloxone was most obvious in relieving brain edema.TNF-αin the injury group was in an upgrading trend at the time of 6h,12h,24h after injury(P＜0.05).Each therapeutic group could inhibit the increasing of TNF-α,and the level of TNF-αat the time point of 6h,12h,24h after injury of rats compared with control group(P＜0.05),which indicated that magnesium sulfate with naloxone can both inhibit the level of TNF-α,and combined medication was better than medication alone. ConclusionIn the acute phase of brain injury of rats in 24 h,the concentration of TNF-αwas in an increasing trend.As TNF-αincreased,the brain edema was also aggravated obviously.Magnesium sulfate and naloxone were used early to decrease the concentration of TNF-αand brain edema,combined medication was most obvious, which indicated that magnesium sulfate and naloxone can both protect the brain injury after trauma,and combined medication was better.
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