A Study On Anti-inflammtory Mechnism Of Total Flavonoid In Leaves Of Ginkgo Biloba In Asthma Mice Model

Bronchial asthma is one of the common and multiple respiratory diseases which was caused by various cells, such as eosinophils, mast cells, T lymphocytes, neutrophils, airway epithelial cells, etc. The cell components were involved in chronic inflammatory airway disease. These cells and cell interactions in a vicious circle so that the airway inflammation persists, as the basic pathology of asthma and recurrent major pathophysiological mechanisms.The leaves and fruit of Ginkgo biloba both have important value in asthma treatment. In Song Dynasty, Ginkgo biloba was used to cure asthma patients in Chinese traditional treatment. Many academicians in the world did a great deal of study about the chemical composition and pharmacological effect which showed that the extract of Ginkgo biloba played an important role in much treatment such as cardiovascular and cerebrovasular disease, hyperlipidemia, respiratory system disease and nervous system diseases. We established the asthma model in BABAL/c mice to to ingredients the total flavonoid in leaves of Ginkgo biloba (FG) on the treatment of asthma in mice inflammatory mechanism, the following major elements:1 The effect on antioxidant capacity of FG on mice asthma modelAsthma in mice was established by ovalbumin (OVA) challenge methods. After intraperitoneal injection of 25 and 50 mg / mL aqueous solution of flavonoids Ginkgo biloba and dexamethasone as the positive control, the content of SOD, MDA, hydroxyl radicals, GSH-Px and NO in mice serum was tested according to Kit. The results showed that FG can improve antioxidant capacity in asthma mice. The content of SOD, MDA, and NO in mouse serum of low-dosage group (116.78±11.57U/mL; 3.25±0.41nmol/mL; 17.71±0.76nmol/mL) was significantly lower than the model of asthma group (130.88±3.60U/mL; 5.31±0.78nmol/mL; 23.03±2.15nmol/mL); the content of hydroxyl radical and GSH-Px (706.90±10.49U / mL; 1396.58±85.14U/mL) was significantly higher than that of asthma model group (679.10±5.55U/mL; 896.12±64.22U/mL), all P <0.05.The content of SOD, MDA, hydroxyl radicals, GSH-Px and NO in mouse serum of high dose treatment group (81.02±4.66U/mL; 1.55±0.39nmol/mL; 719.16±16.71U/mL; 1788.55±75.96U/mL; 13.30±0.51nmol / mL) content closer to the normal group (81.18±7.75U/mL; 1.80±0.47nmol/mL; 740.24±16.59U/mL; 1847.50±84.72U/mL; 13.59±1.94nmol/mL), all P <0.05.2 The effect on inflammatory cell of FG on mice asthma modelEosinopil was an important effector cell in asthma which caused inflammation of bronchial mucosa injury and airway obstruction. The aggregation and degranulation of Eos in airway was the important reason of airway hyperresponsiveness. This experiment study the differential count in BALF and observe the pathology slice of lung and trachea in asthma mice by hematoxylin-eosin staining. The results showed that the number of Eos in BALF of FG treated group can significantly reduce (P <0.01). Pathological biopsy showed that infiltration of inflammatory cells such as lymphocytes in the bronchial and tracheal mucosa and submucosa of model group was notably increased. The bronchial wall thickening, epithelial cell proliferation, basement membrane hyperplasia, smooth muscle hyperplasia, the Endovascular accumulation of secretions, Tracheal mucosa lamina propria and vascular congestion was significantly increased. It also can see more vascular red blood cells. The treatment group have improved compared to the model group.3 The effect on inflammatory cytokines on mice asthma modelThe activation, movement, degranulation and the release of toxic proteins and cytokines in airway inflammation play an important role in tissue damage, airway narrow and the formation of reversible airway remodeling process. The content of PGE2, LTC4 and ECP was tested by enzyme-linked immunosorbent assay (ELISA). The content of LTC4 and ECP of FG treated group was significantly reduced. The difference between the model group were statistically significant (P <0.01). The content of PGE2 of FG treated group was increased, P<0.01. The correlation between Eos number in BALF and the content of PGE2, LTC4 and ECP is obvious (R1 = 0.7113; R2 = 0.6869; R3 = 0.6923, all P <0.01).