Studies On The Chemical Constituents Of Clerodendron Cyrtophyllum Turcz

Clerodendron cyrtophyllum Turcz,a plant widely distributed in the south of China,has been used in traditional Chinese medicine.This plant possesses the function of "heat-clearing and detoxicating,to expel wind-evil and remove wetness".In the research of traditional Chinese medicine,it is available in anti-virus.On the basis of the summary of their chemistry and pharmacology,we have taken a further study in chemical consituents of Clerodendron cyrtophyllum Turcz.From the water extract of the Clerodendron cyrtophyllum Turcz,by solvent extraction,repeated silica gel column chromatography, ODS column chromatography,sephadex LH-20,recrystallization and PHPLC,18 compounds were isolated from Clerodendron cyrtophyllum Turcz.On the basis of physico-chemical characters and spectroscopic analysis,their structures were identified as:acteoside(1),darendoside B(2), p-hydroxyphenethyl-β-D-glucoside(3),phenethyl-β-D-glucoside(4),syringaresinol-4′-O-β-D-glucopyranoside (5),(+)-phillyrin(6),4-hydroxyl-2,6-dimethoxyphenyl-β-D-glucoside(7), vanillic acid(8),gallic acid(9),succimic acid(10),mannitol(11),adenosine(12),β-sitosterol(13). Among them,compounds 1-13 were obtained from this plant for the first time.The fingerprint chromatography of Clerodendron cyrtophyllum Turcz extraction was investigated. Compounds 2,4,5,12,17,the main chemical constituents,were confirmed in the HPLC chromatograph,and the herbal drug Clerodendron cyrtophyllum Turcz were analyzed by HPLC using adenosine and syringaresinol-4′-O-β-D-glucopyranoside as the quality control criterion.The results showed that the average adenosine contents were 0.807 mg/g,the average syringaresinol-4′-O-β-D -glucopyranoside contents were 0.141 mg/g.This research will be providing a preliminary direction for the research and development of Clerodendron cyrtophyllum Turcz.in the future.