A Study On The Association Between The Interleukin-8-251A/T Gene Polymorphisms And Susceptibility To Acute Pancreatitis The Condition Of Patients With Acute Pancreatitis

Objective To investigate the relation of IL-8-251A/T gene polymorphisms in the promoter region and susceptibility to acute pancreatitis(AP)and the condition of patients with AP.Methods The polymorphisms of interleukin-8 gene -251A/T was analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR—RFLP)and DNA sequence analysis methods in patients(n=71)with AP(MAP 36,SAP 35,not SIRS37,SIRS34)and healthy control(n=70).The distribution frequencies of the genotype and allele were compared among different groups.The plasma concentrations of IL-8 was measured by ELISA,and the plasma concentrations of IL-8 was compared among different groups.The patients with SAP were divided into groups on the basis of differet genotypes,and th relative clinical date(the scores of Balthazar CT,APACHEⅡand MODS)were compared among different groups and the score of Balthazar CT and APACHEⅡwere compared between SAPⅠand SAPⅡ.The statistics software SPSS13.0 was used to finish 't' test and 'x~2' test.Results(1)L-8-251A/A +A/Tgenetype frequency of SAP patients was significantly from that of MAP patients,AAgenetype frequency were in AP patients was significantly from thin heathy controls.IL-8-251 genetype frequency of AP patients with SIRS was not significantly different from that of the patients without SIRS.(2) The plasma levels of IL-8 in patients with AP was significantly higher than that in the healthy controls(P＜0.05).The plasmalevels of IL-8 in patients with L-8-251A/A +A/Tgenetype was significantly higher than T/Tgrouo(P＜0.05).(3)In patients with SAP,the scores of Balthazar CT, APACHEⅡand MODS in IL-8 -251A/A+A/T组group was significantly higher than that in IL-8 -251T/T group(P＜0.05).In patients with IL-8 -251T/Z genotype,There was no significant difference in the score of APACHEⅡbetween the first day and the forth to seventh day in hospital(P＞0.05). There was significantly reduction in other groups.The scores of Balthazar CT and APACHEⅡin SAPⅡwere significantly higher than that in SAPⅠ. Conclusions IL-8-251 gene polymorphism is probably related to the development of AP and genetic background charaterized by carrying AA gene maybe increse the risk of AP.It is associated with severity of AP and may worsen AP by carrying AA gene Locus.IL-8-251 gene polymorphism is important in determining plasma IL-8 levels and the plasma IL-8 levels were associated with disease severity of AP.The plasma concentrations of IL-8 was important characteristics to acute pancreatitis(AP).