| Objective: To investigate the effects of recombinant human interleukin(IL)-10 on the cytokines and pathophysiological changes in L-arginine-induced acute necrotizing pancreatitis in rats.Methods: Ninty-two Sprague-Dawley rats were randomly divided into three groups. Group A(n=36) and Group I(n=32) received three intraperitoneal injection of 6% L-arginine (3×1.0mg/g) at hourly intervals. Group I(n=32) was treated with 10,000 unites of intraperitioneal recombinant human IL-10 at the 2nd, 5th and 8th hour after the last injection of L-arginine. Group C(n=24) received saline alone. Rats were killed at the 4th, 12th, 24th and 36th hour after the last L-arginine injection. Serum interleukin-1β, interleukin-6 and amylase were assayed. Pancreatic tissues were fixed in formalin. Histopathological score were recorded according to the edema, inflammation and necrosis of the tissues in three groups.Results: Compare with group C, serum amylase and interleukin-1β , interleukin-6, pancreatic histopathological scores in group A increased significantly after L-arginine injection(P<0.05 or P<0.01). Compared with group A ,the levILs of serum amylase , interleukin-1β ,interleukin-6 in group I were significantly decreased at various time(P<0.05 or P<0.01). Pancreatic...
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