| ObjectiveThe purpose of this thesis is to provide experimental basis for clinical application of iodine -125 seeds implantation by Investigating the therapeutic effect of iodine -125 interstitial brachytherapy seeds on the transplanted liver cancer of BALB/C nude mice and to analyse the influence of doses of radiation to tumor, its surrouding tissue and the blood cells and to analyse the effect of low dose rate and continuous irradiation with iodine -125 seeds in inhibiting proliferation, inducing apoptosis in HEP-G2 cell line and the treatment effect, influence on proliferation, angiogenesis, apoptosis related protein of iodine -125 seeds brathytherapy to liver cancer in nude mice.Methods4x106/0.3m1 HEP-G2 cells were transplanted subcutaneously in the left axillary fossa of BALB/C nude mice, and forty transplanted liver cancer models were produced ,thirty-six fitable models were selected to take an animal experimental research. 14 days later nude mice with a tumor of 6mm or 10mm in mean diameter were produced and separately divided into two different therapeutic dose group and a control group. Each group included 6 nude mice. One iodine -125 seed (surface radioactivity 0.7mci,0.9mci) was implanted into each tumor of mice in 6mm treatment group and the tumor in control group was implanted into One iodine -125 seed (surface radioactivity 0mci );two iodine-125 seeds(surface radioactivity 0.7mci,0.9mci) were implanted into each tumor of mice in 10mm treatment group and the tumor in control group was implanted into two iodine -125 seeds (surface radioactivity 0mci ); Tumors' dimensions of two groups were measured per 7 days and tumor volume inhibition rates were calculated. The blood cells of mice were also observed before and after treatment. After 28 days, nude mice were killed and routine pathological slides of tumor tissue were observed under light microscope to evaluate the degree and range of tumor tissues damaged by iodine -125 seeds. The expression of Proliferating Cell related Nuclear Antigen (Ki67), UEA-1 ,bcl-2 and bax were examined with immunohistochemical methods in six groups. The ratio of bcl-2/bax ,apoptosis by TUNEL way and MVD in six group were calculated.results1. In two weeks followed I125 seeds implantation, there were no volume difference in 0.7mci,0.9mci treatment group tumor, two weeks later the volume in 0.7mci and 0.9mci treatment group tumor in 6mm tumor beagn to reduce ,the control group tumor had been growing . There were significant differences between the 0.7mci ,0.9mci group and the control group xenograft volumes 7 days ,14 days ,21 days and 28 days later after implantation (P= 0.0045,P<0.0001, P<0.0001和P<0.0001,P<0.05,P<0.05,P<0.05,P<0.05).In seven days after I125 seeds implantation, there were no volume difference between 1.4mci,1.8mci treatment group tumor and the control group , There were significant difference between the 1.4mci ,1.8mci group and the control group xenograft volumes 7 days ,14 days ,21 days and 28 days later after implantation (P <0. 05),in addition there were volume difference between 1.4mci treatment group and 1.8mci treatment group。Routine pathological examination showed that dramatically coagulative necrosis and degenerative necrosis of tumor cells at the periphery of active I125 seeds, while there were still viable cells far away from I125 seed.No significant difference of the blood cells in nude mice were observed before and after treatment (P>0.05) 2. Immunohistochemical examination showed that the expression of ki67 in 0.7mci ,0.9mci I125 seed implantation group was"++-++++,++-+++,"while in control group the expression was"+++一+++++".There were significant difference among the 0.7mci,0.9mci groups,the control group.(u= 2.923, 0.025 |
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