Research On The Intervention Of GM6001 In The Traumatic Proliferative Vitreoretinopathy

PURPOSES: To investigate the expression of MMP-2,MMP-9 and TIMP-1, TIMP-2 during the course of tPVR treated with GM6001 and without GM6001, and indicate the difference of ultramicrostructure between them. To explore the potential role of MMP-2, MMP-9 and TIMP-1, TIMP-2 during the course of tPVR and evaluate the interventional effect of GM6001 in tPVR course.METHODS: 180 SD rats were divided randomly into three groups: the control group, the tPVR group, the tPVR treated with GM6001 group. The expression of MMP-2, MMP-9 and TIMP-1, TIMP-2 was analysised by Western Blot on day 1, 3, 7, 14, 21 and 28. The ultramicrostructure of the retina was revealed by the transmission electron microscope.RESULTS:1. The results of Western Blot showed that the expression of MMP-2 in the SD rats'retina of the tPVR group was stronger than other groups at day 3, 7, 14, 21 and 28; the tPVR treated with GM6001 group was weaker than the tPVR group at day 14, 21 and 28d. The expression of MMP-9 in the tPVR group was stronger than other groups at all time; the tPVR treated with GM6001 group was weaker than the tPVR group at day 1, 3, 7, 14 and 21.2. The rate of MMP-2/TIMP-2 of the SD rats'retina in the tPVR group increased at day 14, 21 and 28, and it was lower in the tPVR treated with GM6001 group compared with the tPVR group. The rate of MMP-9/TIMP-1 in the tPVR group increased at all time, and it degraded in the tPVR treated with GM6001 group as compared with the tPVR group.3. The transmission electron microscope showed that the retinal photorecepter cells, the retinal pigment epithelium and the retina inner and outer barriers were damaged in the tPVR group. In the tPVR treated with GM6001 group, the membranous discs of the outer segment of the retinal photorecepter cells were still clear. There were plenty of chondriosome, pinocytosis bullules and smooth endoplasmic reticulum in the cytoplasm, while some of the cristae in the chondriosome were lost. The cell junction was clear and regular. The plasma membrane infoldings of the basilar part of the retinal pigment epithelium in the tPVR treated with GM6001 group were more than that in the tPVR group.CONCLUSION: The disbalance of MMP-2 and TIMP-2, MMP-9 and TIMP-1 involves with the course of tPVR. GM6001 playes an important role in interfering the course of tPVR by regulating the balance of these cell factors and protecting the ultramicrostructure of the retina.