Application Research Of Capillary Electrophoresis In The Analysis Of Traditional Chinese Medicine

This thesis includes the following five chapters:Chapter 1We introduced the application and development of capillary electrophoresis in the analysis of traditional chinese medicine,narrated the background and significance of our study.Chapter 2To establish a method for the determination of imperatorin and isoimperatorin in Radix Angelicae dahuricae and Yuanhu Zhitong Pian by non-aqueous micelle using capillary electrokinetic chromatography. The separation was performed on a uncoated fused silica capillary of 55cm×75μm ID (47cm effective length). 20mmol/L NaH2PO4 +100 mmol/LSDS+80%formamide buffer solution(pH7.12) was selected for the running buffer. The voltage applied was 15kV. The sample was injected by gravity(5s,15cm). The detection wavelength was 254 nm. The internal standard was berberine hydrochloride. The linear ranges of determination for imperatorin and isoimperatorin were 8.0～40.0μg·ml?1(r=0.9987)and 4.0～20.0μg·ml?1(r=0.9990). The average recovery for imperatorin in Radix Angelicae dahuricae was 100.1%,precision of the method was 2.83%(RSD,n=6). The average recovery for isoimperatorin in Radix Angelicae dahuricae was 102.4%,precision of the method was 3.07%(RSD,n=6). The average recovery for imperatorin in Yuanhu Zhitong Pian was101.3%,precision of the method was 2.58%(RSD,n=6). The average recovery for isoimperatorin in Yuanhu Zhitong Pian was 100.2%,precision of the method was 2.64%(RSD,n=6). The method proved to be conveient,rapid and well repeatable,and can be used for quantitative determination of Radix Angelicae dahuricae and Yuanhu Zhitong Pian.Chapter 3To establish a method for the determination of berberine hydrochloride,palmatine hydrochloride,jatrorrhizine hydrochloride and tetrahydropalmatine in Niantong Wan by capillary electrophoresis. The separation was performed on a uncoated fused silica capillary of 55cm×75μm ID ( 47cm effective length ) . 100 mmol/L sodium dihydrogen phosphate+50% ethano(lpH5.70) was selected for the running buffer. The voltage applied was 21kV. The sample was injected by gravity(5s,15cm). The detection wavelength was 211 nm. The linear ranges of determination for berberine hydrochloride,palmatine hydrochloride,jatrorrhizine hydrochloride and tetrahydropalmatine were 8.0～64.0μg·ml?1(r=0.9989),2.5～12.5μg·ml?1(r=0.9997),0.4～3.2μg·ml?1(r=0.9982),0.2～1.6μg·ml?1(r=0.9987). The average recovery for berberine hydrochloride was 103.5%,precision of the method was 2.35% (RSD,n=6). The average recovery for palmatine hydrochloride was 100.2%,precision of the method was 3.07% (RSD,n=6). The average recovery for jatrorrhizine hydrochloride was 102.8%,precision of the method was 3.10% (RSD,n=6). The average recovery for tetrahydropalmatine was 97.8%,precision of the method was 2.71% (RSD,n=6). The method proved to be conveient,rapid and well repeatable,and can be used for quantitative determination of the preparation.Chapter 4To establish a method for the determination of baicalin and chlorogenic acid in Yinhuang Jiaonang and Yinhuang Keli by capillary electrophoresis. The separation was performed on a uncoated fused silica capillary of 55cm×75μm ID (47cm effective length). 40 mmol/L Na2B4O7 buffer solution(pH9.30) was selected for the running buffer. The voltage applied was 15kV. The sample was injected by gravity(5s,15cm). The detection wavelength was 310 nm. The linear ranges of determination for baicalin and chlorogenic acid were 20.2～101μg·ml?(1r=0.9981) and 5.15～25.75μg·ml?(1r=0.9991). The average recoveries of baicalin and chlorogenic acid were 105.0% and 97.2% in Yinhuang Jiaonang,respectively. The average recoveries of baicalin and chlorogenic acid were 101.3% and 96.6% in Yinhuang Keli,respectively. The method proved to be conveient,rapid and well repeatable,and can be used for quantitative determination of the preparation.Chapter 5To establish a method for the determination of baicalein,wogonoside,wogonin and chlorogenic acid in Yinhuang Jiaonang by capillary electrophoresis. The separation was performed on a uncoated fused silica capillary of 55cm×75μm ID (47cm effective length). 40 mmol/L Na2B4O7 + 15 mmol/Lβ-CD buffer solution(pH9.35) was selected for the running buffer. The voltage applied was 12kV. The sample was injected by gravity(5s,15cm). The detection wavelength was 280 nm. The linear ranges of determination for baicalein,wogonoside,wogonin and chlorogenic acid were 2.5～12.5μg·ml?1(r=0.9986),10.0～80.0μg·ml?(1r=0.9981),1.25～10.0μg·ml?(1r=0.9995),and 15.0～120.0μg·ml?1(r=0.9984). The average recovery for baicalein was 104.6%,precision of the method was 2.57% (RSD,n=6). The average recovery for wogonoside was 97.2%,precision of the method was 1.97% (RSD,n=6). The average recovery for wogonin was 98.5%,precision of the method was 2.87% (RSD,n=6). The average recovery for chlorogenic acid was 95.8%,precision of the method was 2.88% (RSD,n=6). The method proved to be conveient,rapid and well repeatable,and can be used for quantitative determination of the preparation.