Study On Capillary Electrophoresis And Its Application In Separateion And Analysis Of Chinese Traditional Medicine Ingredients

In this thesis, the development and application of CE in the analysis ofChinese traditional medicine were reviewed, the basic principle andcharacteristics of CE were introduced; Several techniques capillaryeletrophoresis, including on-line concentration, non aqueous capillaryeletrophoresis, micellar electrokinetic chromatography and capillarysurface dynamical modification were used for separation and determinationof the effective contents of Chinese traditional medicine; Meanwhile, thefollowing aspects were investigated in details.1. A new method was developed for the determination of salicylic acid,cinnamic acid, ferulic acid and vanillic acid in herbal bystacking-non-aquous-capillary electrophoresis with UV detection.Theoperating buffer was methanol, containing 40mmol/L sodiumacetate-2.5mmol/L sodium hydroxide. The UV detection wavelength was225 nm. The linearity ranges of 4 organic acids were 1.38-27.6μg/mL, 0.404-8.08μg/mL, 0.72-18μg/mL, 0.74-29.6μg/mL. Detection limits was 0.069,0.051, 0.107 and 0.089μg/mL for 4 organic acids respectively. This methodcan provide an effective tool for the strict control of these fetal herbalmedicine components.2. A new method for the simultaneous determination of kaempferol andquercetin was established by sweeping micellar eletrokineticchromatography. The operating buffer was composed of 20 mmol/L boricacid,10mmol/L sodium dihydrogen phosphate, 20 mmol/L sodium dodecylsulfate (SDS) and 20％(v/v) methanol with pH 2.30. Up to 200-foldimprovement in concentration sensitivity was achieved by using thissweeping injection technique. The linearity range of kaempferol andquercetin were 1.80-14.4μg/mL, 1.65-13.2μg/mL. The detection limits forkaempferol and quercetin were 0.11 and 0.089μg/mL respectively. Thismethod has been successfully used for the determination of quercetin inHypericum japonicum Thunb. ex Murray.3. A new method for the simultaneous determination of jasminoidin,paeoniflorin and paeonol by micellar electrokinetic capillarychromatography had been proposed. The operating bufer condition was8mmol/L CTAB and 4mmol/L NaOH. The linearity range of jasminoidin,paeoniflorin and paeonol were 20-100μg/mL, 5-80μg/mL, and 2-20μg/mL.This method is simple, rapid and accurate. It had been successfully used forthe determination of jasminoidin, paeoniflorin and paeonol in jiawei xiaoyao pills.4. A new method for the simultaneous determination of shikimic acidand benzoic acid in Illicium verum was established by capillary zoneelectrophoresis. The operating buffer was composed of 50mmol/L sodiumacetate and 0.7mmol/L hexadecyltrimethy-lammonium bromide (20％methanol). The linearity range of shikimic acid and benzoic acid were1-12μg/mL.This method is simple, rapid and accurate. It had beensuccessfully used for the determination of shikimic acid and benzoic acid inIllicium verum Hook.f.5. A new method for the simultaneous determination of ferulic acid andisoferulic acid in Cimicfuga foetida was established by capillaryelectrophoresis. The operating buffer was composed of 20 mmol/L disodiumtetraborate decahydrate and 15％isopropanol. The linearity range offerulic acid and isoferulic acid were 1.44-18μg/mL, 1.8-180μg/mL. Thismethod is simple, rapid and accurate. It had been successfully used for thedetermination of ferulic acid and isoferulic acid in Cimicfuga foetida.