The Study Of Genes HLA-DRB1*1101 And HLA-DRB1*1201 Which Relate To Non-or Poor Hyporesponse To Hepatitis B Vaccine And HBsAg Infection

Objective: To primarily confirm the association between HBsAg infection, HLA-DRB1*1101, HLA-DQB1*1201 in combination with the non-or poor hyporesponsse to hepatitis B vaccine.Methods : A study which included 75 non-or poor hyporesponders and 485 responders from 653 children who were subjected to a standard course of immunization with recombinant hepatitits B virus vaccine. All subjects were study with their health investigated file. Genes HLA-DRB1*1101 and HLA-DRB1*1201 were detected with ploymerse chain reaction-sequence specific primes(PCR-SSP). The data were analysed by SPSS 13.0 software.A study about 628 children which included 37 HBsAg positive children. All subjects were subjected to a standard course of immunization with recombinant hepatitits B virus vaccine. All subjects were study with their health investigated file. Genes HLA-DRB1*1101 and HLA-DRB1*1201 were detected with ploymerse chain reaction-sequence specific primes(PCR-SSP). The data were analysed by SPSS 13.0 software. Results: (1)Univariate analysis indicated that dosage 10ug(OR=0.265, 95%CI 0.141～0.498 ), and gene HLA-DRB1*1201 were associated with non-or poor hyporesponse.(2)The results of multivariate logistic regression also showed that dosage 10ug(OR=0.265, 95%CI 0.141～0.498 )and gene HLA-DRB1*1201(OR=4.612, 95%CI 1.55～13.726)were associated with non-or poor hyporesponse. (3)Univariate analysis indicated that HBsAb positive(OR=0.128, 95%CI 0.064～0.256) and dosage 10ug(OR=0.274, 95%CI 0.098～0.767)were associated with HBsAg infection . (4)The results of multivariate logistic regression showed that Gene HLA-DRB1*1101 and HBsAb positive(OR=0.128, 95%CI 0.064～0.256) were associated with HBsAg infection .Conclusion: Gene HLA-DRB1*1201 was associated with non-or poor hyporesponse. Gene HLA-DRB1*1101 was associated with HBsAg infection .