Observing Reversing Effect Of Quercetin Associated Antisense Oligodeoxynucleotide Against Surviving On The Multi-drug Resistance Of Human Hepatocellular Carcinoma SMMC7721/ADM Cells

Objective To explore the reversing effect of quercetin associated antisense oligodeoxynucleotide against Survivin(ASODN)on the multi-drug resistance of human hepatocellular carcinoma SMMC7721/ADM cells.Methods Choiced hepatocellular carcinoma sensitive cells SMMC7721 and multi-drug resistance cells SMMC7721/ADM,by the MTr,RT-PCR and immunocytochemistry to observe their drug resistance against four kinds of chemotherapy drug and reversing effect of quercetin and antisense oligodeoxynucleotide against surviving on multi-drug resistance of SMMC7721/ADM ceils.Results Quercetin associated antisense oligodeoxynucleotide against survivin obviously deduced IC50 of SMMC7721/ADM cells to four kinds of chemotherapy drug than only used one of them(P＜0.05);only used quercetin and quercetin associated antisense oligodeoxynucleotide against survivin could obviously deduce the expression of MDRI-mRNA and P170 and MRP-mRNA and P190 of SMMC7721/ADM cells(P＜0.05). Conclusion Quercetin associated antisense oligodeoxynucleotide against survivin obviously reversed the drug resistance against four kinds of chemotherapy drug of SMMC7721/ADM cells than only used one of them.The way may be that quercetin could restrain the expression of MDR1-mRNA and MRP-mRNA,so the production of P170 and P190 were deduced,then produced the reversing effect on drug resistance.Antisense oligodeoxynucleotide against survivin could not produce the effect by this way.