| Objective:To discuss the following things by establishing a simpleness and controled soft tissue explosive wound mold.Firstly,in order to providing local debriedement basis by probing into the specialty of infection after soft tissue explosive under field environment in Lan Zhou area.Secondly,for to providing basis of rational use of drugs via investigating the spectrum and drug sensitivity of explosive injury in soft tissue in Lan Zhou areas.Thirdly,would healing effect of external-using of traditional chinese materia.Fourly,the contrast of methods wound treatment for searching a kind of wound dealing method which is fast,convenient and effective.Medthods:Experiment 1:5 San-yuan healthy piglets were slected,and two hindlimbs in one were paired.According to paired design,those objects were assigned to nature vulnerated group and degermed vulnerated group,muscle tissue of explosive would were removed at 4h,7h,9h,12h,16h,24h,48h after explosive injury respectively and were made into homogenate.Then,with releasing peuring platform methods bacteria were counted.The bacterial spectrum and drug sensitivity were performed.Meanwhile,the environmental bacteria were detected.Experiment 2:8 San-yuan healthy piglets were slected,liberality buttocks were vulnerated by paper detonator after anaesthesia and fixation,then,those 16 wounds were divided into control group and Sheng ji yu hong gao group and Tuo guan san group.Dressing change and drawing materials for observing under microscope and mensurating of proline content and mensurating of cell proliferation index of wound and wound area and depth were all done respectively.At last,by variance analysis better effect of group was slected.Results:Experiment 1:1.Bacterial measurement in nature vulnerated group reached 10~5 cfu/g at 12h after explosive injury,while degerm vulnerated group reached 10~5 cfu/g at 16h.2.Ba-cillus subtilis and surface bacteria were found in group A and Bacillus subtilis and Klebsiella pneumoniae and Proteus mirabilis were found in group B.Gram-positive bacteria were drug resistant to most antibiotics and were only sensitive to quinoxalone antibiotics and vancomycin.Gram-negative bacteria were sensitive to third-generation cephalosporin and aminoglycoside antibiotics.Experiment 2:Compared with control group,the difference of content of proline and proliferation index and wound depth was significant at 4,7,10,14 days respectively after vulnerating,and wound area was at 10,14days(P＜0.05).Tuo guan san group compared with Sheng ji yu hong gao group difference of proline content from seventh and proliferation index from fourth and wound from tenth were significant(P＜0.05).Conclusions:1.Infection point of explosive injury in soft tissue in Lan Zhou area was at 12h after explosive injury.Infection point of degerm vulnerated group was later 4h than not.The best point of debridement of explosive wound in soft tissue in Lan Zhou area was in 12 hours after vulnerate.2.Early infection of explosive soft-tissue wound was closely related to environmental bacteria,therefor,debriedement should be done early and the range of skin disinfection should be extensive and thoroughly as far as possible.Early application of antibiotics should give priority to quinolones and third-generation cephalosporin and aminoglycoside antibiotics.3.To compare with the control,Yu hong sheng ji gao and tuo guan san had some effect on the controling of infection in explosive wound about soft tissue and promoting granulation growth and collagen synthesis and wound contraction.4.Curative effect of tuo guan san on explosive wound about soft tissue was superior to Sheng ji yu hong gao;Tuo guang san could accelerated would healing and stabilize wound condition.
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