Observation Of T-cell Subsets In The Cases With And Without Response To HBsAg Vaccination

OBJECTIVE:To investigate the T-cell subsets in the cases with and without responses to HBsAg vaccination,and to explor the relationship between immune respone to HBsAg and activation of T-cell subsets.METHODS:Peripheral blood from 18 responders to HBsAg vaccination(responders),22 nonresponders and 10 naive nonimmunized healthy controls(controls)was analyzed for the expressions of CD69, CD25,CD4,CD8 in peripheral blood mononuclear cell,and CD4+CD25+regulatory T cell frequency was aslo analyzed by Flow cytometry.RESULTS:1.The percentage of CD69+CD4+T cells in CD4+ T cells from responders,nonresponders and controls were 1.81±1.33%,0.64±0.48%,and 0.56±0.39%,respectively.CD69 expression on CD4+T cells in responders was increased as compared to controls as well as nonresponders(p＜0.01),but no difference between nonresponders and controls(p＞0.05).The percentage of CD69+CD8+T cells in CD8+ T cells from responders was significantly lower than that of nonresponders(0.43±0.25%vs.3.05±1.69%,P＜0.01)and controls (0.43±0.25%vs.3.11±2.41%,p＜0.01).2.The percentage of CD3+CD4+T cells in CD3+ T cells from responders,nonresponders and controls were 50.84±6.36%,53.67±6.68%,and 51.02±9.30%,respectively.There was no difference statistically between responders and controls.The same result could be found between responders and nonresponders(p＞0.05).CD8 expression on CD3+ T cells in responders,nonresponders and controls were 41.02±4.41%, 42.69±7.35%,and 42.36±8.34%,respectively.There was no difference statistically between responders and controls as well as nonresponders (p＞0.05).3.The percentage of CD4+CD25+ regulatory T cell in CD4+ cells from responders,nonresponders and controls were 0.59±0.46%,0.77±0.40%,and 1.30±1.44%,respectively.There were no significant statistical difference between them(p＞0.05).CONCLUSION:1.Insufficient early activation of Th concerned with nonresponse to HBsAg vaccination.2.The increasing activation of Ts may relevant to HBsAg nonresponse.3.The CD4+CD25+ regulatory T cell may play an important role in the negativity regulation of response to HBsAg vaccination.We might go no the further research with large sample to conform it.