Reunion Mutagenesis Of Ultraviolate Rays And Diethyl Sulfate To The Ecoli From The Intestinal Tract Of Uremic Patient

Objective:Using reunion mutagenesis of ultraviolate rays and diethyl sulfate to elevate urea and creatinine degrading ability of Ecoli from intestinal tract of patient with uremia , accordingly provide new strain for the entero-bacteria therapy of chronic renal failure.Methods:1. Isolate Ecoli from enterobacteria of patient with chronic renal failure,assess and test if there is pathogenicity.2. Determine basic ability of degrading urea and creatinine about Ecoli from uremic patient's intestinal tract, compared to normal Ecoli.3. Mutagenesis:make growth curve of the Ecoli from the intestinal tract of the patient with uremia→definite growth logarithmic phase→determine the action time and intensity of ultraviolet rays( UA rays)→prepare Ecoli suspension→ultraviolet mutation→dilute the bacteria suspension and cultivate on the screening plate→detect urea and creatine degrading ability of the screening bacteria suspension→repeat screening the bacteria which can decrease the creatine concentration in uremic patient circulation→Amplificate the strain which obtain maximum decrease in the concentration of the urea and creatinine solution after degrading→definite growth logarithmic phase→diethyl sulfate mutation(the induction of mutation process is the same to the uv mutation )→repeat screening and then investigate the stability of thescreening bacteria.Result:1. Isolate pure Ecoli from the uremic patient intestinal tract,presume no pathogenicity through initial asess and test.2. There is decrease which has no statistical significance in the residual concentration of the urea and creatinine degraded by Ecoli from intestinal tract of patient with uremia.3. Reunion mutagenesis of ultraviolate rays and diethyl sulfate to the Ecoli from the intestinal tract of the patient with uremia elevate about 32.5% of the urea degrading ability and about 7.5% of the creatine degrading ability;4. Repeatedly serial subcultivation,the stability of the urea and creatinine degrading ability about the screening strain is below the mark.Conclusion:1. Isolate a no pathogenicity Ecoli from enterobacteria of patient with chronic renal failure,and it has some ability of degrading urea and creatinine.2.The Ecoli from enterobacteria of patient with chronic renal failure with obviously elevated ability of degrading urea and creatinine was screened after reunion mutagenesis of ultraviolate rays and diethyl sulfate., but the stability of the Ecoli after mutagenesis needs elevation.