| Objective: Cervical cancer is one of the common gyneco- logic malignancies, whose incidence is only after breast cancer. Before occurrence of this malignancy, it underlies a long time of intraepithelial neoplasia. In recent years, the mobility of cervical intraepithelial neoplasia (CIN) and cervical cancer raises grad- ually, especially, in young women. In 1995, International Center for Research on Cancer (IARC) clearly put forward that human papillomavirus (HPV) infection is a major risk factors of cerv -ical cancer. The research of molecular mechanisms'found that HPV-E6 protein E6 protein plays its role in advancing cancer with the p53which is one of the cycle regulatory factors. But in pathogenesis, the studies suggests that infection with HPV alone is not sufficient for the development of CIN or cervical cancer and underscores the importance of other cofactors, such as cell cycle disorder, apoptosis inhibitory mechanism and so on. CyclinE and Survivin are focus of the current research. In this study, we use immunohistochemistry and flow cytometry method (FCM) to detect the expression CyclinE, Survivin and HPV16-E6 protein in normal cervix, chronic cervicitis, CIN and cervical cancer tissues, approaching the relationships of cervical cancer age, clinical stage, degree etc. Clinical features with these three factors, and research these three factors'correlations. Likethis, it may provide a new way for cervical cancer'mechanisms, provide a theoretical basis for the early diagnosis of cervical cancer and provide new targets for the prevention and treatment of early cervical cancer.Methods1 The expression of CyclinE, Survivin and HPV16-E6 was examined by s-p immunohistochemical stain (IHC) in 10 cases of normal cervical tissues, 30 cases of chronic cervicitis, 24 cases of CIN and 48 cases of cervical cancer tissues.2 The expression of CyclinE, Survivin and HPV16-E6 in 10 cases of normal cervical tissues, 12 cases of chronic cervicitis, 15 cases of CIN and 25 cases of cervical cancer tissues were quantitatively examined by flow cytometrye (FCM).3 SPSS 11.5 was applied to analyze the results of experiment.Results:1 The expression of CyclinE in normal cervical tissues, chronic cervicitis, CIN and cervical cancer increased gradually, and there was statistical significance between 4 groups (P<0.01). Between CIN and cervical cancer group, there was no significant difference (P>0.05). Combined these two groups, then in contrast to CIN, cervical cancer group, there were significant difference (P<0.01, P<0.05). Between CIN and cervical cancer group, there was significant difference (P<0.05). In cervical cancer group, the expressions of cyclinE were correlated with the pathological type, histologic grade, clinical stage and lymph node metastases (P<0.05), but not with age, tumor size, latero-uterus or haemal infiltration and the depth of invasion (P>0.05).The expression of CyclinE was also detected by FCM. The flurescent index of normal cervical tissues, chronic cervicitis, CIN and cervical cancer were 0.98±0.06, 1.00±0.04, 1.40±0.12 and 1.68±0.15. There was statistical significance between 4 groups (F=131.95, P<0.01).2 The expression of Survivin in CIN, carcinoma group were obviously higher than that normal cervical tissues and chronic cervicitis (P<0.01). In cervical cancer groups, the expressions of Survivin were correlated with the clinical stage, lymph node metastasis, and histologic grade (P<0.05), but not with age, pathological type, latero-uterus or haemal infiltration, tumor size and the depth of invasion (P>0.05).The expression of survivin was also detected by FCM. The flurescent index of normal cervical tissues, chronic cervicitis, CIN and cervical cancer were 0.99±0.09, 1.03±0.02, 1.33±0.03 and 1.93±0.24. There was statistical significance between 4 groups (F=121.24, P<0.01).3 The expression of HPV16-E6 protein in normal cervical tissues, chronic cervicitis, CIN and cervical cancer increased gradually, and there was statistical significance between 4 groups (P<0.01). Normal cervix contrast to chronic cervicitis group, there was no significant difference (P>0.05). Combined these two groups, then contrast to CIN, cervical cancer group, there are significant difference (P<0.01, P<0.05). Between CIN and cervical cancer group, there was no significant difference (P>0.05). In cervical cancer groups, the expressions of HPV16-E6 were correlated with the age and pathological type (P<0.05), but not with, histologic grade, clinical stage and lymph node metastasis tumor size, latero-uterus or haemal infiltration and the depth of invasion (P>0.05).The expression of HPV16-E6 was also detected by FCM. The flurescent index of normal cervical tissues, chronic cervicitis, CIN and cervical cancer were 0.96±0.08, 1.02±0.02, 1.35±0.14 and 1.43±0.16. There was statistical significance between 4 groups (F=47.31, P<0.01).4 The Spearman rank correlation analysis method was used to analyze the correlation of CyclinE, Survivin and HPV16-E6 in cervical cancer. The expression of CyclinE was positively related to that of Survivin (r=0.459, P<0.01). The expression of CyclinE was positively related to that of HPV16-E6 (r=0.522, P<0.01). The expression of Survivin was positively related to that of HPV16-E6 (r=0.404, P<0.01).Conclusions: 1 The expression of CyclinE, Survivin and HPV16-E6 in normal cervical tissues, chronic cervicitis, CIN and cervical cancer increased gradually. The abnormal expression of them may be involved in the occurrence and development of cervical cancer. Maybe the detection of the expression these three factors'will be use for the early diagnosis of cervical cancer2 The expressions of cyclinE were correlated with the pathological type, histological grade, clinical stage and lymph node metastasis. The expressions of Survivin were correlated with the clinical stage, lymph node metastasis and histological grade. They would be possible to become effective monitoring indexs that predict the invasion and prognostic of cervical cancer.3 The higher expression of HPV16-E6 protein infects <45 patients of cervical cancer more than older patients, and the expression of HPV16-E6 is related to Cervical squamous cell carcinoma'occurrence.4 It shows that cyclinE, survivin and HPV16-E6 are in administrative relation, cooperated the process of cervical cancer genesis and growth.
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