Anti-Tumor Activity Of 2, 6-Diisopropyl Phenol Derivatives

PART 1 EFFECTS OF 2,6-DIISOPROPYL PHENOL DERIVATIVES ON HUMAN BREAST CANCER CELLSObjective:To observe the effects of 2,6-diisopropyl phenol derivatives on proliferation and apoptosis of human breast cancer cells.Methods:Breast cancer cell lines MDA-MB-231 were cultured in vitro.There were 8 groups in the experiment:cell control group,solvent control group(0.5%DMSO),paclitaxel control group,different concentration experimental group of 2,6-diisopropyl phenol derivatives at 50μM,100μM,200μM,400μM and 600μM.Each experimental group continued to be cultured for 24 hours after intervention in order to be investigated.Optimal cell innovulation concentration and culture time were chosen with the application of MTT assay;detecting morphologic change and proliferation condition of tumor cells in each group and then observing every group's apoptosis of tumor cells with flow cytometer.Results:(1)The optimal innoculation concentration of human breast cancer cell line MDA-MB-231 was found to be 5.0×10~4/ml and the administration timing of drug should be 24 hours after inoculating cells with MTT assay. (2)There was no morphologic change or proliferation condition change of tumor cells in cell control and solvent control groups within 24 hours.(3)Lots of necrotic cells were observed in hydrocamptothecin control group,compared with 2,6-diisopropyl phenol derivatives at 400μM and 600μM concentration groups,the difference was not significant (P＞0.05).(4)No morphologic change or proliferation condition change of tumor cells was found in 2,6-diisopropylphenol derivatives at 50μM and 100μM concentration groups within 24 hours.Part of dead tumor cells were observed and some cells underwent apoptosis in 200μM concentration group,compared with 50μM and 100μM groups,the difference was remarkable(P＜0.05);In 400μM and 600μM concentration groups,there were a big amount of dead tumor cells after 24 hours,lots of tumor cells underwent apoptosis,compared with 50μM,100μM and 200μM groups,the differences were quite significant(P＜0.01),group comparsion between 400μM and 600μM showed that the difference was not significant.Conclusion:2,6-diisopropylphenol derivatives have the activity to resist human breast cancer cell line MDA-MB-231. PART 2 EFFECTS OF 2,6-DIISOPROPYLPHENOL DERIVATIVES ON HUMAN NEUROBLASTOMA CELLS AND HEPATOMA CARCINOMA CELLSObjective:To detect effects of 2,6—diisopropylphenol derivatives on human neuroblastoma cells and hepatoma carcinoma cells.Methods:Human neuroblastoma cell lines and hepatoma carcinoma cell lines were cultured in vitro sepreately.Paclitaxel control group in part 1 changed to hydroxycamptothecin control group in part 2.The other of experimental groups,concentration and time controlled,and detecting part same as part 1.Results:(1)The optimal innoculation concentration of human neuroblastoma cell lines and hepatoma carcinoma cell lines was found to be 5.0×10~4/ml and the administration timing of drug should be 24 hours after inoculating cells with MTT assay.(2)There was no morphologic change or proliferation condition change of tumor cells in cell control and solvent control groups within 24 hours.(3)Absorbance values of neuroblastoma cell group,200μM,400μM and 600μM groups had significant difference with that of 50μM and 100μM groups(P＜0.01).Group comparsion between 200μM and 400μM groups showed no sifnificant difference(P＞0.05).Comparsion between 400μM and 600μM groups had obvious difference.Compared with 200μM and 400μM groups,hydroxycamptothecin control group had no remarkable difference(P＞0.05),however,when compared with 600μM group,the difference was significant(P＜0.01)(4)Hepatoma carcinoma cell group and 50μM group had no significant difference with cell control group and solvent control group in absorbance values(P＞0.05).Absorbance values of 100μM,200μM,400μM and 600μM groups had very significant difference with that of 50μM group and contol group(P＜0.01).There was significant difference between 600μM group and 100μM,200μM,400μM groups(P＜0.01).Compared with 100μM and 200μM groups,hydroxycamptothecin control group had no obvious difference(P＞0.05),when compared with 400μM group,the difference was significant(P＜0.05),as compared with 600μM group,the difference was quite remarkable(P＜0.01)(5)Group comparsion between two kinds of tumor cells at 100μM concentration showed significant difference(P＜0.01)Conclusion:2,6-diisopropyl phenol derivatives have the activity to resist human neuroblastoma cells and hepatoma carcinoma cells,but the effect of anti-hepatoma carcinoma cell is better.