Changes Of Hsp70 And DCs Expression In Model Of Rabbit Liver VX2 Carcinoma Before And After Radiofrequency Ablation Combine Transcatheter Arterial Chemoembolization And High Freguency Hyperthermia

Objective: To evaluate the effect of rabbit liver vx2 tumor mode treated by RFA ,HFH and TACE.Methods: To build the model of rabbit liver tumor in transplantation ,treated by RFA,TACE and HFH. To divide into groups base on the different way ,group A : RFA+TACE+HFH,group B: RFA+HFH,group C: RFA+TACE Group D : TACE+HFH. To take suction the blood of ear veins at 1st,7th ,14th day for detecting blood serum Alanine aminotransferase ( ALT).Meanwhile take the spleen ,all of the collection affixed in 4% paraformaldehyde and paraffinembedded in program.Half of each group experiment animal sacrificed in random at 7th day and others at 14th day.To detecte HSP70 and S-100βpositive dendrtic cells in immunohistochemistry.Results: (1).ALT is step up obviously in first day in all of groups, The range of group A is most width in all and recovered most slowly. The range of group B is most short and recoverd quickly.(P<0.05).(2)Hsp70 express in liver,the range of group A is most width in all of groups in 7th day and in 14th day. group D is the most shortest and the lowest.(P<0.05).(3).DCs express in spleen there is no discrepancy in group A and group B (P>0.05),meanwhile,there has no discrepancy in 7th day and 14th day.the expression in group C and group D is lower and recovered more quickly(P<0.05).Conclusions: RFA+TACE+HFH can stimlulate the expression of Hsp70 and DCs more efficiency. To enhance the immunopotency of antitumors ,but they can do more hurt on liver function. It need we have more attention when we use.