Detection Of Perforin And Granzyme B In T Lymphocyte Subsets In Protection To Tuberculosis Infection

ObjectiveTo investigate the levels of perforin and granzyme B expressions in T lymphocyte subsets in the protective immune against tuberculosis.MethodsThirty mice were randomized into two groups:namely normal control group(N group) and tuberculosis group (A group)( n=15 each). 0.1mg standard person type mycobacterium tubereulosis were injected into the tai1 vein of mice in A groups. Six weeks after infection, percentage of perforin and granzyme B expressions in T lymphocyte subsets from peripheral blood were detected by flow- cytometry intracellular stain.Results1. The percentage of perforin-experssed CD4+ T lymphocytes (mean 21.92) and granzyme B-experssed CD4+T lymphocytes (mean 14.69) were no significant difference than that of in health contorls (mean 20.56, 12.98) (P >0.05).2. The percentage of perforin-experssed CD8+ T lymphocytes (mean 17.33) was no significant difference than that of in health contorls (mean 14.55) (P >0.05), but granzyme B-experssed CD8+T lymphocytes (mean 80.49) in tuberculosis group was higher than that of in health contorls (mean 51.16) (P <0.01).3. The percentage of perforin-experssed DPT lymphocytes (mean 40.23) was no significant difference than that of in health contorls (mean 40.16) (P >0.05), but the percentage of granzyme B-experssed CD8+ T lymphocytes (mean 64.93 ) in tuberculosis group was higher than that of in health contorls (mean 32.99) (P <0.05).ConclusionsIt showed that granzyme B expression in CD8+T and DPT lymphocytes were increased in the protective immune against tuberculosis, but granzyme B-expressed CD4+T lymphocytes and perforin expression in CD4+T,CD8+T and DPT lymphocytes were no significant difference.