Correlation Between The Insertion/Deletion Polymorphism Of Angiotensin Converting Enzyme Gene And Early Onset Preeclampsia

Objective: Examine the Insertion/Deletion polymorphism of angiotensin converting enzyme(ACE) gene, so that to detect the correlation between the Insertion/Deletion polymorphism of ACE gene and early onset preeclampsia and to investigate the relationship between the Insertion/Deletion polymorphism of ACE gene and the severity of early onset preeclampsia.Methods: 1. Study object: One hundred and twenty preeclamptic patients including 60 early onset cases and 60 later onset cases and 60 normal pregnant women were recruited from Women and Children Health Hospital of Jiangxi Province during September 2006 and September 2007.2. Method: The Insertion/Deletion polymorphism of ACE gene was amplified by polymerase chain reaction (PCR). The frequencies of ACE genotypes and alleles were counted and compared between preeclampsia and control group respectively. Statistical analysis was conducted to determine the correlation between the gene polymorphism and early onset preeclampsia.Results: 1. The distribution of ACE genotypes in preeclampsia group and normal pregnant group were according with Hardy-Weinberg equilibrium law.2. The distribution of ACE genotype between early onset preeclampsia group and late onset preeclampsia group was different (P<0.05)and the frequency of allele was significantly different(P<0.01). The distribution of ACE genotype between early onset preeclampsia group and normal pregnant group was different (P<0.05)and the frequency of allele was significantly different (P<0.01) . There were no differences in the distribution of ACE genotype and the frequency of allele between late onset preeclampsia group and normal pregnant group(P>0.05).3. The difference in the distribution of ACE genotype between preeclampsia with renal dysfunction group and preeclampsia without renal dysfunction group was significantly(P<0.05), but there was no significant difference in the frequency of allele between two groups(P>0.05). The plasma creatinine in ACE genotypes' group was different, the plasma creatinine of DD genotype group was the highest in three genotypes(P<0.05).4. The incidence of renal dysfunction in early onset preeclampsia was significantly higher than late onset preeclampsia(P<0.01).Conclusions: 1. The insertion/deletion polymorphism of ACE gene associated with early onset preeclampsia. The DD genotype was susceptible to early onset preeclampsia.2. The insertion/deletion polymorphism of ACE gene associated with the renal dysfunction of preeclamptic patients. The DD genotype was a high risk predict for preeclampsia complicated with renal dysfunction.3. The DD genotype maybe contributes to the occurrence and development of early onset preeclampsia through influencing renal function.