Extraction And Structural Of Antitumor Activity From Euphorbia Ficheriana

Euphorbia fischeriana is a perennial herbaceous plant which belongs to the Euphorbia family. In recent years, it has been reported that the extracts from Euphorbia fischeriana Steud had a significant antitumor activity. The aim of this dissertation is to separate the antitumor constituents from Euphorbia fischeriana,clarify their antitumor pharmacological activities and identify their structures by spectroscopic analysis together with physicochemical data.In this paper, the chemical constituents from Euphorbia fischeriana have been protested systematiincally by means of tube-test. It is found that Euphorbia fischeriana contain phytosterols and terpenoids, flavonoids, coumarin, lactones, saponin, etc. The optimal operation parameters of refluxing extraction process were chosen by tests. The optimal operation parameters: the rate of solid to liquid is 1:6, refluxing extracted 4h in 85℃water bath. On the condition, 95% ethanol extract, 70% ethanol extract and water soluble extract were isolated by means of solvent extraction. Then 95% ethanol extract was partitioned between petroleum ether, CH3CH2OOCH2CH3, acetone and methanol to give four fractions. It was found that the petroleum ether and CH3CH2OOCH2CH3 extracts strongly inhibited cell proliferation of human cancer cell lines with a significant dose-dependent response by a CCK-8 (Cell Counting Kit-8) assay. The inhibitory rates of the petroleum ether extract at a dose of 100μg/ml against human stomach cancer SGC-7901, human colon cancer HT-29, human breast cancer are 56.48%, 52.61%, 45.37% respectively which is such similar to the inhibitory rates of the CH3CH2OOCH2CH3 extract as 52.19%, 48.73%, 50.12% . Furthermore, the CH3CH2OOCH2CH3-partitioned extract was purified by means of column chromatography and multiple-crystallization. Finally, two compounds were obtained. By chemical action, physical constant and spectral analysis, the structure of compound X-1 was identified asβ-sitosterol and the structure of X-2 was identified as lupeol.