| Background:The specificity of acupoints and mechanism of acupuncture have become the focus of research recently. The specificity of acupoints was summarized from practice of long term. At present, the clinical effect of acupuncture has been admitted by the WHO. In order to prove the specificity of acupuncture and explore the route of acupuncture treating diseases, researchers from home and abroad have completed a great many experimental studies through modern scientific methods and achieved good results. For exploring the specificity of irritation from acupuncture and its mechanism further, we measured blood pressure of hypotension rats treated by acupuncture on twelve yuan-source points and changes of some vasoactive substance's content. We hope to find objective evidence from two aspects, macroscopic and microcosmic, so as to provide experimental basis for the specificity of acupoints.Objective:We researched the specificity of acupoints through observing changes of blood pressure of hypotension rats treated by acupuncture on twelve yuan-source points and non-points. We also discussed the mechanism of acupuncture's effect of boosting pressure, through detecting the content of NO and NOS from peripheral blood serum and expression of ET-1 from thoracic aorta of rats, which belonged to the teams of best effect. Materials and methods:We chose 140 male Wistar rats (clean animals, 180—220g), which were divided randomly into two groups according to primal blood pressure, with 6 rats in the control group and 134 rats in the treatment group. The rats in the treatment group were given suspension of Compound Reserpine Tablets by intragastric administration (8ml/kg), twice a day. Two weeks after intragastric administration, the rats whose blood pressure was less than 80mmHg were considered success in molding. Then according to blood pressure, the successful animal models were divided randomly into 14 teams: Shenmen team, Daling team, Hegu team, Yangchi team, Wangu team, Taiyuan team, Taibai team, Jinggu team, Taixi team, Chongyang team, Taichong team, Qiuxu team, model team and non-point team, with 6 rats in each team. We did nothing to the rats in the control group and model team, but do acupuncture therapy to the rats in the twelve-yuan-source-point teams and non-point team. We chose points of both sides, after insert in the body we used mild reinforcing-reducing method, continuously stimulated the points for 5 minutes. Everyday after treatment we measured blood pressure of tail artery of each rats 5 times and calculate the mean value. After the experiment, we detected the content of NO and NOS from peripheral blood serum and expression of ET-1 from thoracic aorta of rats, which belonged to the teams of best effect. All data were analyzed by SPSS13. 0. Results:1. After treatment, the blood pressure of twelve-yuan-source-point teams and non-point team were compared with that of the model group, they were statistically significant (P< 0.01), which indicated that acupuncture had effect of boosting pressure. The blood pressure of twelve-yuan-source-point teams and non-point team were statistically significant (P<0. 01), which indicated that yuan-source points had effective specificity. Among the twelve yuan-source points, Shenmen, Daling, Hegu and Yangchi had the best curative effect. These four teams were not statistically significant (P>0.05), which means they had the same curative effect. Taiyuan and Taichong's effect took the second place, they were not statistically significant (P>0.05), which means their curative effect were mainly equivalent. Wangu, Taibai and Taixi's effect took the third place, they were not statistically significant (P> 0.05) too, which means their curative effect were on the same level. Jinggu, Chongyang and Qiuxu had the worst effect, they were not statistically significant (P>0.05), which means their effect were similar.2. The content of NO and NOS in peripheral blood serum of Shenmen, Daling, Hegu and Yangchi team declined remarkably comparing with model team (P< 0.01). The data of these four teams and that of non-point team were statistically significant (P<0. 01) too. These four teams were not statistically significant (P>0.05).3. The expression of ET-1 in thoracic aorta of Shenmen team reinforced, and showed a strong positive reaction (P<0. 05).Conclusions:1.Twelve yuan-source points' effect of boosting pressure had point specificity. Among the twelve yuan-source points, Shenmen, Daling, Hegu and Yangchi had the best curative effect. Taiyuan and Taichong' s effect took the second place. Wangu, Taibai and Taixi's effect took the third place. Jinggu, Chongyang and Qiuxu had the worst effect.2. Acupuncture had good regulative effect to NO/NOS system of hypotension rats. Acupuncture could lessen NO's relaxation effect to vascular smooth muscle cells through lowering the content of NO and NOS in peripheral blood serum. Maybe contracting blood vessel to boost pressure is one aspect of the mechanism.3. Shenmen' s effect of boosting pressure was probably related to reinforced expression of ET-1 in thoracic aorta of rats.
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