| Lonicera japonica Thunb., was common traditional chinese medicine having the property of clearing heat and toxin, antibacterial and antiphlogistic, protecting liver and cholagogic, an evergreen liana plant with its stems, leaves and flowers as medicine. The chemical constituents of the stems and leaves of L. japonica were similar to the flowers of L. japonica, most of which with strong pharmacological activities. This thesis based on preliminary systematic test with L. japonica as the research object, using spectrophotometry to determine the distribution of effective composition in aerial part of L. japonica. The antibacterial activities of different solvent extracts of the ethanol extract of the stems and leaves of L. japonica were studied by the filter paper method. In addition, the chemical constituents in the stems and leaves of L. japonica were separated and purified by chromatography, and the structure of the monomeric compounds were identified. The main results are as follows:1. The chemical constituents of the stems and leaves of L. japonica were studied through the pre-testing by the tube method, compared with the flowers of L. japonica. The results showed that the stems and leaves of L. japonica had the similar chemical composition with the flowers of L. japonica. Both of them contained phenolic, tannins, flavonoids, flavonoid glycosides, sugar, glycoside, saponin, steroidal, terpenes, amino acids, peptide, proteins, fats and volatile oil constituents, and didn't contain alkaloids, anthraquinone, anthraquinone glycoside, coumarin, coumarin glycoside, cardiac glycosides constituents, may contain esters and lactone components.2. The contents of phenolic acids, flavonoids and soluble sugar of the stems and leaves of L. japonica were determined by UV spectrophotometry, compared with the flowers of L. japonica and the flowers of wild L. japonica. The results showed that the content of total phenolic acid, total flavonoids and total soluble sugar in the stems and leaves of L. japonica were 12.74mg·g-1,21.64mg·g-1,77.40mg·g-1 respectively. The content of total phenolic acid were in the order: the stems and leaves of L. japonica < the flowers of the wild L. japonica < the flowers of the L. japonica, and the content of total phenolic acid in the stems and leaves of L. japonica accounted for 32.53 percent of the flowers of the wild L. japonica and 41.11 percent of the flowers of the wild L. japonica. The content of flavonoids were in the order: the flowers of the wild L. japonica < the stems and leaves of L. japonica < the flowers of the L. japonica, and the content of flavonoids in the stems and leaves of L. japonica accounted for 18.07 percent of the flowers of the wild L. japonica and 259.16 percent of the flowers of the wild L.japonica. The content of soluble sugar were in the order: the flowers of the wild L. japonica < the flowers of the L. japonica < the stems and leaves of L. japonica, and the content of flavonoids in the stems and leaves of L. japonica accounted for 148.82 percent of the flowers of the wild L. japonica and 529.05 percent of the flowers of the wild L. japonica. The results showed that phenolic acid and flavonoids distributed more in the flowers of L. japonica than in the stems and leaves of L. japonica. Comparing with the flowers of wild L. japonica, the content of active components in the flowers of L. japonica were improved, which showed that introduction and cultivation of L. japonica could improve the internal quality.3. The antibacterial activities of different solvent extracts of the stems and leaves of L. japonica were studied by the filter paper method. The results showed that different extracts perform antibacterial activity in 60mg·ml-1. The antibacterial effects of the ethyl acetate extraction against Phytophthora Capsici, Fusarium oxysporum.f sp. cucumerinum and Staphylococcus aureus were the best from the whole aspect, and the minimal inhibitory concentration were 20mg·ml-1, 10mg·ml-1, 15mg·ml-1 respectively. Inhibitory effect of alcohol part was next to the ethyl acetate part, which showed a strong inhibition against Fusarium oxysporum.f sp. cucumerinum.The water part showed faint antibacterial activities against Fusarium oxysporum f. sp. fragariae and Penicillium, medium antibacterial activities against others. The chloroform part showed no activity against Fusarium oxysporum f.sp. fragariae, and faint antibacterial activities against Penicillium, medium antibacterial activities against others. The activities of the petroleum ether part was the weakest, showed no activity against Phytophthora Capsici and Penicillium, faint antibacterial activities against Fusarium oxysporum f. sp. fragariae and Fusarium oxysporum.f sp. cucumerinum, medium antibacterial activities against others.4. Six compounds were isolated from the ethanol extract of the stems and leaves of L. japonica by CC, TLC, recrystallization and so on, which had been proved to be single compound by purity testing. Four of them were identified by modern spectroscopic techniques (IR, EI-MS, 1HNMR, 13CNMR, DEPT) as follows: compound H2 (βsitosterol), compound IV (daucossterol), compound V (ethyl caffeate),compound VI (chlorogenic acid). This thesis studied the chemical constituents of the stems and leaves of L. japonica through the pre-testing, and determined the contents of phenolic acids, flavonoids and soluble sugar of the stems and leaves of L. japonica. The distribution of chemical constituents in the stems and leaves of L. japonica was preliminary definite. The antibacterial activities of the stems and leaves of L. japonica was also preliminary definite through the studies on the antibacterial activities of different solvent extracts of the stems and leaves of L. japonica. Six compounds were isolated from the ethanol extract of the stems and leaves of L. japonica, which enriched the research contents, and offered the theory basis for rational exploitation and utilization.
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