The Relation Between REG 1A Gene Expression And The Clinicopathologic Parameters In The Patients With Primary Gastric Carcinoma

ObjectiveTo investigate the relation between expression of REG 1A gene, its clinical features and the survival rate in the patients with primary gastric cancer.Methods(1) The technology of extraction RNA from paraffin-embedded materials. (2) Reverse transcriptase-polymerase chain reaction (RT-PCR). (3) Immunohistochemical techniques. (4) SPSS statistic software analyzed the experimental results.ResultsUsing RT-PCR and immunohistochemical techniques, REG 1A mRNA and protein expression were investigated in 235 primary gastric carcinoma samples. Those were revealed in the results. First, the positive REG 1A mRNA is 78% (183/235) of primary gastric carcinoma which revealed by RT-PCR analysis. Moreover, patients with REG 1A mRNA-positive differentiated adenocarcinoma were found to have a significantly poorer prognosis compared with REG 1A mRNA-negative tumor patients. REG 1A mRNA is closely linked to the infiltrative growth pattern and with signet cell and poorly differentitated adencarcinoma. And the incidence of venous invasion of REG 1A mRNA-positive tumors was significantly higher than that of REG 1A mRNA-negative tumors. And second, the positive rate of REG 1A protein is 31.1% (73/235) in 235 patients with primary gastric carcinoma. REG 1A protein expression in infiltrating tumors was found to be significantly higher compared with localized tumors (P<0.05). REG 1A protein expression in the cytoplasm of signet ring cell carcinoma tumors was significantly stronger than in nonsignet ring cell tumors. Moreover, the patients with REG 1A protein-positive differentiated adenocarcinoma were found to have a significantly poorer prognosis compared with REG 1A protein-negative tumor patients. The incidence of venous invasion of REG 1A protein-positive differentiated adenocarcinoma was significantly higher than that of REG 1A protein-negative tumors.-ConclusionsThe results of the experiment were demonstrate that the expression of the REG 1A gene is closely related to the infiltrating property of primary gastric carcinoma, signet ring cell and poorly differentiated adencarcinoma, significantly enhanced the risk of venous invasion of REG 1A protein-positive differentiated adenocarcinoma and may be as a prognostic indicator of differentiated adenocarcinoma of the stomach in clinic diagnosis.