Expression And Clinical Significance Of Mta1 Gene In Non-Small Cell Lung Cancer

Objective:In order to investigate the relationship between the expression of matastasis - associated gene 1 and non-small cell lung cancer invasion and metastasis, we researched expression of matastasis - associated gene 1(mtal)in primary carcinoma tissue,paracarcinous tissue,normal tissue and corresponding lymph nodes of non-small cell lung cancer and lung innocence tissue.Methods:Optimal conditions of nested reverse transcription polymerase chain reaction(RT-PCR)were found out;then the expression of mtal gene mRNA in 42 samples of primary carcinoma tissue,paracarcinous tissue,normal tissue and corresponding lymph nodes was compared with 20 lung innocence tissue at semi-quantitative level and the results were compared with clinical pathologic data.Result:Average expression of the mtal gene in NSCLC primary carcinoma tissue (1.50±0.26)and lymph nodes with metastasis(1.88±0.35)was higher than that in normal tissue(1.02±0.17)and lung innocence tissue(0.90±0.15)(P＜0.01). Average expression of the mtal gene in NSCLC primary carcinoma tissue(1.50±0.26)was higher than that in paracarcinous tissue(1.09±0.16).Average expression of the mtal gene in lymph nodes with metastasis(1.88±0.35)was higher than that in those without metastasis(1.40±0.36)(P＜0.01).The frequency of mtal overexpression in NSCLC tissue correlates with clinical staging,T staging and N staging;the frequency of mtal overexpression was 45.2%(19/42)in NSCLC tissue; the frequency of mtal overexpression was 84.2%(16/19)in lymph nodes with metastasis and was not related with age,gender and type of tumor. Conclusion:Our data suggests that the overexpression of the mtal gene correlates with invasion and metastasis of NSCLC.A high expression of mtal mRNA may be a potential indicator for assessing the malignancy and metastasis of NSCLC.