C-reactive Protein Induced Vascular Endothelial Cells Apoptosis And The Expression Of P-selectin

Objectives: To observe the effect of C-reactive protein(CRP) on the cell apoptosis and the expression of P-selectin by ECV304 cells.Methods: Through incubation of ECV304 cells with different concentration of CRP(0ug/ml,5ug/ml,25ug/ml,50ug/ml,100ug/ml)for 24hours, to observe the dose effect of CRP on ECV304 cells. Through incubation of ECV304 cells with CRP (50ug/ml) for different time (3hours,6hours,12hours,24hours), to observe the time effect of CRP on ECV304 cells. Cells apoptosis analyzed by flow cytometry and P-selectin measured by Enzyme-linked immunosorbent assay(ELISA).Results①.Compared with control group(0ug/mlCRP), CRP caused a significant increase in the rate of apoptosis at a dose as low as 5ug/ml(12.16%±3.69%,P<0.01) and reached peak value at 50ug/ml (27.91%±3.11%,P<0.01) . The rate of apoptosis was elevated as early as 3 hours(2.71%±0.93%,P<0.05) after CRP(50ug/ml) incubation and reached peak value at 24hours(27.91±3.11,P<0.01).②.Compared with control group(0ug/mlCRP),CRP caused a significant increase in the expression of P-selectin at a dose as low as 5ug/ml(15.93ng/ml±3.77ng/ml,P<0.01) and reached peak value at 100ug/ml (86.35ng/ml±7.35ng/ml,P<0.01) . The expression of P-selectin was elevated as early as 3 hours(34.33ng/ml±5.01ng/ml , P<0.01) after CRP(50ug/ml) incubation and reached peak value at 6hours((79.28ng/ml±7.63ng/ml,P<0.01).Conclusion: CRP induced ECV304 cells apoptosis and upregulate the expression of P-selectin in a dose and time dependent manner.