The Research Of The Differential Expression Proteome Of The Left Ventricular Muscle Of The Rat During The Progress Of The Athletic Heart Remodeling

Objective: The protein is the executant of the organism function, the athletic heart remodeling and the pathology heart changes come of the changes of the protein, and there is an essential difference between them. our study probed into the differential expression and the law of the left ventricular muscle proteome during the progress of the medium intensity exercise from the sight of quantitative and qualitative, and seeking out the objective proteins who had a significant function during the progress of the athletic heart remodeling, and providing new gists for discovering the mechanism of the athletic heart remodeling, and providing reference for the sport healing or the adjunctive therapy of the chronic cardiac disease.Methods:Using 54 male SD rats as our study object, and dividing them into exercise and parallel control groups according to their avoirdupois (each 3 groups,n=9) . The exercise groups were killed after anaesthesia together with their parallel control group after 4, 8 and 12 weeks medium intensity (70-80%VO_2max) training respectively after 6h at the last training, weighing heart of each rat, preparing for whole protein samples of the left ventricular muscle, and separating the protein samples by utilizing two-dimensional gel electrophoresis, and analysing image and collecting data by using PD-Quest. The alternatively objective protein spots whose differential expressive volume≥10 folds were selected as candidate spots, and using them to have an in-gel enzymologic extraction and MS/MS identification.Results:The heart weight index of the exercise groups increase 18. 5%,34.5% and 36.6% (p<0.05) respectively after 4, 8, 12 weeks exercise. 584±23 and 555±33 protein spots are detected in the 4 weeks exercise groups and control groups respectively, there 13 points whose differential expression volume are≥10 folds in it, 4 spots are upregulated and 9 spots are downregulated. 499±29 and 495±26 protein spots are detected in the 8 weeks exercise groups and control groups respectively,the proteins whose differential expression volume≥10 folds are the same as the 4 weeks. 578±27 and 587±26 protein spots are detected in the 12 weeks exercise groups and control groups respectively, there are 4 points expressed≥10 folds in it, they are new and upregulated. Taking out 5 of 17 alternatively objective protein spots, and identified 4 of them, 78kDa glucose-regulated protein ( GRP78 ) , Enoyl-CoA hydratase ( ECH ) and ATP synthase subunit alpha are upregulated after exercise, ATP synthase coupling factor 6 ( CF6 ) and an unknown protein whose Mr is 116kDa are downregulated after exercise.Conclusions: (1) The rat heart of the exercise groups increased after 12 weeks' medium intensity training. (2)During the remodeling progress of the test groups heart, the proteome of the left ventricular muscle has a notable differential expression change after 4 weeks' training, and the proteome has an adaptive changes after 8, 12 weeks' training. (3)Using the medium intensity training to have a heart remodeling, the training rat hearts have a well adaptive changes. this can improve the level of cardiac muscle energy metabolism, enstrengthen the ability of cardiac muscle stress reaction and adaption, and meliorate the blood pressure adjustment abilities. (4)we succeed in filtrating 4 objective proteins which are not mentioned in the sports medicine area at present, they are 78 kDa glucose-regulated protein, Enoyl-CoA hydratase, ATP synthase subunit alpha and ATP synthase coupling factor 6,etc. this work establishes a prophase work basic to have a further study on the athletic heart.