| ObjectiveTo investigate the effects of Tempol on aortic function and remodeling in renovascular hypertensive rats.MethodsThe 2 kindey 1 clip hypertensive rats were prepared in male wister rats,then rats were randomly divided into three groups: control rats(n=6), hypertensive rats(n=6) and hypertensive rats treated with Tempol (1mmol/L)in drinking water(n=6), After 8 weeks treatment.BP blood plasma angiotensinⅡ( AngⅡ) , nitric oxide(NO),8-iso-PGF2αlevel were determined. Isometric tension change of aortic rings was recorded;RT-PCR was used to measure the expression of p22phoxmRNA of aorta;Histologic study of aorta was exammed using light microscope .ResultsHypertensive rats had highter BP, AngⅡ, 8-iso-PGF2α, media wall,media wall/ lume (W/ L) (P<0.01);lower NO (P<0.01)when compared with control rats. The expression of p22phoxmRNA in hypertensive rats was significantly higher than that in control rats. Endothelium dependent vasodilatation was significantly reduced(P<0.01) in hypertensive rats compared with control rats.After 8 weeks treatment of Tempol, BP,8-iso-PGF2α, media wall,media wall/ lume (W/ L)were significantly lower(P<0.01); NO were significantly higher; endothelium dependent vasodilatation was significantly recovered when compared with hypertensive rats. There were no differences in AngⅡbetween hypertensive rats and hypertensive rats treated with Tempol.ConclusionAngⅡ, NO ,oxidative stress play important roles in the aortic remodeling in renovascular hypertensive rats. Tempol can reduce BP, media wall,media wall/ lume (W/ L) adjustment NO ,oxidative stress wihout influencing AngⅡ. Therefore, NO, but not changes in AngⅡ, plays a major role in the blood pressure-lowering effects of Tempol. Tempol also can reduce media wall,media wall/ lume (W/ L) improve endothelium dependent vasodilatation in renovascular hypertensive rats. |
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