Purification Of Saponins And The Establishment Of Fingerprint From Momordica Charantia L.

Momordica charantia L. was Cucurbitaceae momordica plant.It lived up to its Bitter Melon name for all parts of the fruit tastes very bitter. Bitter Melon possess various efficiencies such as clearing away heat and toxic materials, removing pathogenic heat from heart and improving activity of sight , decreasing blood sugar, antibacterial, antiumor, antivirus and immunomdulatior. It was used as vegetables as well as medicine. The purification of Saponins from Momordica charantia L. has been studied in this paper, and established the fingerprint of it. In this paper, as follows:Macroporous resins adsorption were used to purify the total saponins from Momordica charan -tia L.The static adsorptive and desorptive capacities of five different types of macroporous resins with different physical properties for saponins had been studied to find a suitable resin for it's purification. Then, the dynamic adsorption and desorption of the choosed resin had been studied. Results showed that resin AB-8 had the best adsorptive and desorptive capacities for the saponins, The best elution was 80% (v/v) ethanol. When the chromatography column's size is 2×60cm, the optimum purification condition was: the slight acidified extract solution(pH6-7)containing 15mg/m -L saponins poured on at 2.5mL/min. The desorption rate would reach 90.7% when adsorbed by 6B -V 80%(v/v) ethanol.The content of saponins would reach 63.2% .Total saponins was proceed silica gel column chromatography, and then washed by chloroform and methanol with different polar, obtained two components after repetition silica gel column chro -matography and crystallization marked a and b. Libermann-Buehard and the bubble experiments characterized them as saponins. Saponins from Momordica charantia L. had the similar structural as Rg1 from Ginseng and for control in the same chromatographic conditions, these two component -s had a similar peak time as well as Rg1 from Ginseng, To further inferred for Bitter Melon saponin. When conditions for the commencement of chloroform: methanol(8:1), Rf values were 0.43 and 0.16.The HPLC fingerprint of different originals Bitter Mellon had been researched to reflect the overall types of saponins, and by HPLC-UV method to determine the optimum conditions, Chromat -ographic column: Zorbax SB-C18 (150×4.6mm,5μm); Column temperature: 25℃; Wavelength: 209nm;Flow rate:1.0mL/min; Inject volume:20μL; Mobile phase: water(A)-acetonitrile(B) gradient elution: 0～15min, A:70%～98%, B:2%～30%; 15～55min, A:40%～70%, B:30%～60%; 55～65min, A:20%～40 %, B:60%～80%.To analyzed the fingerprint of Bitter Mollen saponins by chosing a reference material had a relatively large area,stable and a peak time of about 42.058 min in the chromatogram. Identified a total of 8 peaks, and a similarity calculation of the fingerprint, generated the control fingerprint and obtained a satisfactory results. The established HPLC fingerpri -nt of a higher degree of stability and reproducibility, and provided a means to the evaluation of Mo -mordica charantia L., Conducive to the further development of saponins from Momordica charant -ia L. standards and the control of products.