Effects Of The Resistant Exercise On Free Radical Metabolism Level In Skeletal Muscle Of Aging Rats Induced By D-galactose Treaded

The poor life quality of old people has something to do with the decline of the muscle strength. Sarcopenia which relates to aging is the main cause of muscle strength decline. The cardinal symptom of Sarcopenia is the decline of both muscle quality and muscle strength. The decline of muscle strength increases the possibility of muscle injury, especially the injury in a tumble. In this case, the old have to suffer a decline of life quality and afford a high health protection expense. The effective measure to counteract sarcopenia is over-load resistant exercise. The study focuses on the change of free radical metabolism in skeletal muscles under the condition of developing resistant exercise in the process of aging. In one aspect, to discover the effect of resistant exercise on body free radical metabolism; in another occasion, to discover the effect of resistant exercise on free radical metabolism in skeletal muscles of aging body. It' s time to supply a practical suggestion to do resistant exercise on the point of view about free radical metabolism in skeletal muscles.The study "adopts ladder crawling training to simulate" resistant exercise and continuous large amount of D-galactose injection to simulate the fast-aging process. Two dozen of eight-week-old male Sprange-Dawley rats are required and they are randomly divided into four groups: the young rest group (YR), the aging rest group (AR), the young exercise group (YE), the aging exercise group (AE). The aging groups are injected D-galactose once a day according to their weights(125mg/kg) and they should be weighted every day. The young groups are injected physiological salt injection by rate. The training can be recognized as a tail load crawling ladder training. Each training includes 2 rounds and each round includes 3 times of crawling ladders. The training begins at 4 pm every Monday,Wednesday and Friday and lasts for 10 weeks. 48 hours after the last training, the rats should be all decapitated and be anatomized rapidly. Separate the skeletal muscles and preprocess them in liquid nitrogen, then preserve them in refrigerator at -80 degree centigrade. After homogenating the skeletal muscles, we detected cytoplasm' s MDA,protein carbonyls level,CuZn-SOD,GXH-PX and the expression of the CuZn-SODmRNA,GXH-PXmRNA.Experiment results:(1) The measuring of the rat' s MDA level of both rectus femoris and vastus lateralis : the aging exercise group' s MDA level is significantly higher than the aging rest group' s (P<0. 05).(2) The measuring of the rat' s oxidative carbonyl protein level of both rectus femoris and vastus lateralis: the aging exercise group' s oxidative carbonyl protein level and the aging rest group' s oxidative carbonyl protein level are not significantly different in statistics (P>0. 05).(3) The measuring of the rat' s GSH-PX active level of rectus femoris: the aging exercise group' s GSH-PX level is significantly higher than the aging rest group' s (P<0. 05). The differences of GSH-PXmRNA level of rectus femoris among groups: the aging exercise group' s GSH-PXmRNA level is significantly higher than the aging rest group' s (P<0. 01). The measuring of the rat' s GSH-PX active level of vastus lateralis : the aging exercise group' s GSH-PX active level and the aging rest group' s GSH-PX active level are not significantly different.(4) The measuring of the rat' s SOD active level of rectus femoris: the aging exercise group' s SOD active level is significantly higher than the aging rest group' s (P<0. 05). The differences of SODmRNA level of rectus femoris among groups: the aging exercise group' s SODmRNA level is significantly higher than the the aging rest group' s (P<0.01).The measuring of the rat' s SOD active level of vastus lateralis: the aging exercise group' s GSH-PX active level and the aging rest group' s SOD active active level are not significantly different(P>0.05). Conclusion:The study shows that the crawling ladder training accelerates the lipid peroxidation of both rectus femoris and vastus lateralis of aging rats induced by D-galactose treaded. But it doesn' t prove effective in changing oxidative carbonyl protein level of rectus femoris and vastus lateralis. Besides, the crawling ladder training improves the antioxidant capacity of rectus femoris and vastus lateralis according to the measuring of cytoplasm' s GSH-PX active level,SOD active level and GSH-PXmRNA level,SODmRNA level.