The Influence Of 1, 2, 4-TCB On Mice's Anti-Oxidation System

PREFACE1,2,4 -TCB has pervaded in natural environment and industrial production. It will cause a series of problems due to its longevity and amassment. The EPA has added it into the environmental pollutant list.So many experts have done a lot of research on the toxicity of 1,2,4 - TCB, with the advancement of the research on ROS and diseases , demonstrating that free radical is closely relevant to various toxin injury. ROS is produced in body, which can have lipid peroxidation and do harm to the bio - function of cell membrane leading to injury to the organism.This research is to determine the oxidative damage to mice blood, heart, brain, liver and kidney due to different scales of acute intoxication, and to observe the subacute pathological change with them, and to provide the reference for the establishment of 1,2,4 - TCB hygienic standard.Materials and Methods1. Grouping and intoxication of experiment animals.1.1. 40 mice, halfmale, weight 25 ?g, divide into 4 groups, each group with 10 mice, one control group and 3 experiment groups, intoxicated by subcutaneous injection. The control group is injected with 0. 9%NaCl. The dosage of experiment group are 1382. 2mg/kg, 2764. 4mg/kg and 4146. 6mg/kg respectively. Inject them once. 24 hours after intoxication, anesthetized with ether, and then take blood sample after picking their eye balls. Kill the mice by dissociation of vertebrae, take out the heart, brain, liver and kidney, clean them with 0. 9% NaCl, dry them with filtrated paper and then keep them freezed.1.2. 16 mice, divide into 4 groups randomly with 4 in each group, one control group, 3 experiment groups. The dosages for experiment groups are 1382.2mg/kg, 2764.4mg/kg, 4146.6mg/kg respectively. The control group is injected with 0. 9%NaCl, intoxicated 4 times with the interval of 4 days. Kill the mice after the 4th time by dissociation of vertebrae, take out the liver and the cortex of kidney and make pathologic sheets of LM and EM.2. Observation standard and measurement.2. 1. Weight precisely before measuring and prepare tissue emulsion . Measure LPO in tissue and serum by TEA, SOD in tissue and blood by Nitrite - Ki, and GSH - Px by DTNB, T - SH and NP - SH by Ellman's method. Determine Hb by methemoglobin cyanogeniza-tion. Determine tissue protein by biuret reaction.2.2. LM; conventional Formalin 10% fixation, paraffin embed-ding, HE stain; EM: 2.5% dipentaldehyle primarily fixation, 1% einsteinium acid secondarily fixation and study the intracellular structural change by perspective EM.Result1. The measurement of LPO, SOD, GSH - Px, T - SH, NP - SH in blood and tissue.24 hours after ih. 1 ,2,4 - TCB the content of LPO in the 3 experiment groups in brain and the mid, high dosage groups in liver is obviously higher than that in the control groups repectively, and there is remarkable difference between the content of SOD, NP - SH in the 3 experiment groups in brain, the content of T - SH, PB - SH with low dosage of intoxication, the content of GSH - Px with high dosage of intoxication and those of the control groups; the content of NP - SH and GSH - Px in liver with mid, high dosage are lower greatly than that of the control group while the content of SOD in high dosage shows an obvious increase against its control group; In kidney the content of GSH - Px in the 3 experiment groups and the content of NP -SH in the low dosage of intoxication are remarkable lower than those in the control groups. In blood, the content of GSH - Px in the 3 experiment groups decrease compared with the control group, while the content of T - SH and PB - SH in the mid dosage intoxication increase obviously. All the results above show that the function of peroxidation with lipid of 1,2,4 -TCB has been enhanced after it has entered the body, and it also causes the change of anti - oxidase in blood and tissue accordingly.2. Histo - pathological morphological observation.(1) LM:liver: hepatic cells in the control groups have rarely hydropic...