The Autocrine Phenomenon Of Vascular Endothelial Growth Factor And Its Impacts On Tumor Cells Proliferation In Glioma

Objective: To determined the autocrine phenomenon of vascular endothelial growth factor (VEGF) and its impacts on tumoral cells proliferation in glioma.Methods:â‘ Immunohistochemistry (IHC) was used for determined of VEGF, Vascular endothelial growth factor receptors(VEGFR1/VEGFR2) and Ki-67 expression in brain glioma.â‘¡Immunohistochemistry double staining method was used for determined VEGFR1 and Ki-67 co-expression and VEGFR2 and Ki-67 co-expression in glioma.â‘¢The expression of VEGFR1 and VEGFR2 were measured by image analysis technique.Results:â‘ The positive expression of VEGF, VEGFR1 and VEGFR2 was tested not only in cytoplasm and basement membrane of vascular endothelial cells (EVC) and nuclear but also in cytoplasm and membrane of glioma cancer cells. The VEGF, VEGFR1 and VEGFR2 protein positive-expression correlated significantly with glioma histological grade (P=0.013, r=0.331;P=0.000, r=0.748;P=0.000, r=0.750), and they expressed in the same glioma tissue domain.â‘¡The positive expression of Ki-67 was tested in nuclear of glioma cancer cells, and its expression correlated significantly with glioma histological grade (P=0.000).â‘¢There were co-expression of VEGFR1 and Ki-67, VEGFR2 and Ki-67 in the same tissue domain of some glioma slice. The co-expression of VEGFR1 and Ki-67 was not significantly agreement (Kappa=0.092, P=0.605), while the co-expression of VEGFR2 and Ki-67 was significantly agreement (Kappa=0.543, P=0.001).Conclusions:â‘ This study suggests that there is VEGF autocrine phenomenon in brain glioma.â‘¡Glioma tumor cells proliferation by VEGF autocrine function, especially by VEGF-VEGFR2 pathway.