The Influence Of Batroxobin Administrated In Different Times On Neuroprotective Effects In Treatment Of Cerebral Ischemic Reperfusion Injury

Stroke is one of the most threatening diseases for human health. The patients are always afflicted with functional disorder of movement, sense,cognition and self-care in early stage after stroke.If the functional disorder cannot be effectively treated in time,it will influence the quality of patients′life and become a burden to society and patients′families.At present,the remedy and rehabilitation therapy constitute the methods in treatment of the patients after stroke.Thrombolytic therapy is regarded as an effective way to treat ischemic stroke nowadays.Studies have found that batroxobin does something good in neuroprotection and it has some effect on thrombolysis.Batroxobin could directly affect plasma fibrinogen to form non-bind mono or multiple fibrin which could be easily cleaned by fibrinolysis system,and could promote the t-pA releasing from the endothelial cells,enhance the effect of t-pA and lower the activity of plasminogen activator inhibitor.It could decline the plasma fibrinogen levels and the blood viscosity.The total dose of batroxobin on protection of forebrain ischemia-reperfusion injury in experiment hasn't been reported,thus,this study may give the optimal dose according to corresponding animal experiment.The previous results had showed that Bcl-2 have the role of reducing cell apoptosis but Bax have the opposite effect.There are many animal experiments about brain protection of batroxobin.Hence we examined the expressions of Bcl-2,Bax in different times by intraperitoneally injection,and explain probable mechanism and relationship between times and efficiency of batroxobin to protect brain neurons.There are three sections in this project:1.To study the total dose of batroxobin to protect forebrain ischemia in gerbils 2.The changes of ultrastructure in hippocampal pyramidal in cerebral ischemic reperfusion in gerbils.3.The probable mechanism of batroxobin to protect brain neurons.The results manifested that the quantity of apoptotic neurons in IR group was significantly more than that in other groups,and the quantity of apoptotic neurons in five-time group is significantly less than that in three-time group and in four-time group(P＜0.05),but no significant difference was found between five-time group and six-time group (P＞0.05).The ultrastrctural changes in five-time group were lighter than that in three-time group and four-time group,but no significant difference was found between five-time group and six-time group.From the above results,we reckon here that the optimal times of batroxobin to protect forebrain ischemia-reperfusion injury in gerbils is 5 times.Thereafter gerbils were divided into four groups:normal group,IR group,three-time group,four-time group,five-time group.We used immunohistochemistry technology to accumulate the positive cells of Bcl-2,Bax after forebrain ischemia-reperfusion injury in different group. The result is that with the times of batroxobin used increased,the number of bcl-2 positive cell was gradually increased.but the number of Bax positive cell was gradually decreased with the times of batroxobin increased.Reasonably we think that the neuroprotective effect of batroxobin is achieved by alleviating Bax expression and upgrading the Bcl-2 expression.