The Dynamic Observation Of The Expression Of P-Selectin In Cerebral Ischemia/Reperfusion Rat Model And The Influence Of Batroxobin

OBJECTIVE: To dynamically observe the expression of P-selectin on vessel walls in the ischemia/reperfusion rat model and the influence of batroxobin to the P-selectin expression and the ultrastructures of endothelial cells in ischemic focal tissues.METHODS: To build the ischemia/reperfusion rat model; to measure the P-seletin expressions on vessel walls in the focal ischemic cerebral tissues respectively 1 hour, 3 hours, 6 hours, 12 hours,24 hours, 2 days, 3 days, 4 days, 7 days, 14 days after the reperfusion by immunohistochemical method and the influence of batroxobin; to observe the protective effects of batroxobin to the ultrastructures of endothelial cells in the ischemic focus with the electromicroscope.RESULTS: P-selectin expression on vessel walls gets the firstupregulation one to three hours after the reperfusion and the second one 4 days after the reperfusion. Batroxobin may decrease the expression at the first(p<0.001) and fourth day(p<0.05) statistically, batroxobin may ameliorate the changes of the ultrastructures of endothelial cells and vessels in the ischemic focus.CONCLUSIONS: There is two phases of upregulation of the expression of P-selectin on vessel walls in the cerebral ischemia/reperfusion focus .As an adhesion molecule ,P-selectin plays an important role in the ischemia/reperfusion progress. Batroxobin may decrease the expression of P-selectin on vessel walls and show its protective effects on the structures of the endothelial cells.