The Study On Microsomal Triglyceride Transfer Protein-493G/T Gene Polymorphism In Non-diabetic Patients With Coronary Heart Disease

Background: Coronary heart disease (CHD) is a polygenic inheritance disease. It is a leading cause of death for adults in many countries. Lipid metabolic disturbance is the primary and independent risk factor for coronary heart disease. Genetic variant related with the lipid metabolism will lead to lipid metabolic disturbance, thus promote the development of coronary heart disease. Recently it is reported that the T-variant of the MTP promoter, MTP-493 G/T, might lower cholesterol but increase the risk of coronary heart disease. Also it is indicated that the MTP-493 G/T polymerphism can influence the level of lipids in blood plasma significantly in diabetic, and simultaneously their complication increases. Now there are few studies reported the relationship between coronary heart disease and the MTP-493 G/T polymorphism . Objective: To investigate the relationship between the coronary heart disease and the microsomal triglyceride transfer protein (MTP)-493G/T gene polymerphism . Methods: This study enrolled 335 non-diabetic patients with coronary heart disease(CHD) and 206 control subjects without CHD. The genotype and allele frequencies of MTP-493 G/T were detected by means of polymerase chain reaction-restricted fragments length polymorphism( PCR-RFLP). Results : The first frequency of MTP-493G/T genetic variation was GG genotype, the second was GT genotype and the third was TT genotype in Han population of China. Both the TT genotype frequency (0.06) and the "T" allele frequency (0.19) in patients with coronary heart disease were higher than those in the control subjects (0.03,0.12) (P<0.05) respectively. There was no relationship between MTP -493 G/T genotypes and the coronary vessels diseased. The TT genotype had a higher TG and LDL-C level than GG genotype (P<0.05). In multiple logistic regression analysis, the TT genotype odds ratio for coronary heart disease was 2.14(95 % CI1 .22～6.56, P= 0.014). Conclusion: The TT genotype of MTP-493G/T genetic variation increases the risk of coronary heart disease, independent of lipid and lipoprotein factors.