The Relationship Between Smad4, CyclinD1, P21^waf1 Protein Expression And Clinicopathology Features In Gastric Carcinoma As Well As Patient Survival

Objective To evaluate the expression of Smad4,CyclinD1,p21^waflprotein and MVD, MLD in gastric carcinoma,cancer-adjacent tissue,lymph nodes and determine the relationship between them and tumor clinicopathological features as well as patient survival.Materials and methods 200 surgically resected gastric carcinoma samples during 1999-2004,56 cancer-adjacent tissues,55 lymph nodes and 30 normal gastric mucosa samples were collected.Tissue microarrays was used to determine Smad4,CyclinD1,p21^waflprotein expression and MVD,MLD by immunohistochemistry S-P(Streptavidin-Peroxidase).Software SPSS15.0 was used to analyse data.Results 1 Positive rate of Smad4 in 200 gastric carcinoma tissues is 67.0% (134/200),CyclinD1 and p21^waflare 56.5%(113/200),59.5%(119/200),respectively. No correlation between Smad4 and cyclinD1 by spearman's rank correlation analysis in gastric carcinoma(r=-0.092,P=0.195),while significant correlation were observed in Smad4 and p21^waflexpression(r=0.179,P＜0.05);there is no relationship between CyclinD1 and p21^waflexpression(r=-0.046,P=0.518).No correlation between Smad4,p21^wafland MVD/MLD,CyclinD1 expression associates with MLD,but not MVD.MVD relates with MLD(P＜0.01).2 Smad4 expression is found to be statistically associated with tumor different -iation status and Lauren's types(P＜0.01),and CyclinD1 is associated with age, tumor differentiation status and Lauren's types(P＜0.05),while p21^waflassociated with tumor differentiation status,Lauren's types and lymph node metastasis(P＜0.01).MVD is relates with tumor differentiation status and the depth of tumor invasion(P＜0.05);MLD affects tumor sizes,differentiation,lymph node metastasis and Lauren's types(P＜0.05).3 Positive expression of Smad4 is related with prognostic survival analysed by Kaplan-Meier and Log-rank test(χ²=4.090,P=0.043),but not an independent prognostic factor.Lymph node metastasis and the depth of invasion are independent prognostic factors.Conclusions 1 Smad4 expression rate reduces in gastric carcinoma,and its abnormal expression is related with differentiation and Lauren's types,this indicates that expression of Smad4 influence tumor differentiation;p21^waflexprssion reduces in gastric carcinoma,too,and which is correlates with differentiation, Lauren's types and the age.Its abnormal expression may rezult in tumorigenesis; CyclinD1 appears to be overexpressed in gastric carcinoma,and it is relates with lymph node metastasis,differentiation and Lauren's types.MVD/MLD level rises in gastric carcinoma,and the level,which is related with the bio-behaviors of gastric carcinoma closely,affects the tumorigenesis and development of gastric carcinoma greatly.2 Smad4 is correlates with p21^wafl,which indicates that reduced expression of smad4 leads to abnormal expression of its downstream gene p21^wafl.MVD correlates with MLD and,they interact in gastric carcinoma.3 Survival rate in positive expression of Smad4 surpasses the negative express -ion.Positive expression of Smad4 is a favorable prognostic factor,but not an independent one in patients with gastric carcinoma.