| Objective In the present study,we investigated whether splenectomy leads to postoperative cognitive dysfunction(POCD)in aged rat model undergoing water maze and observed the changes of cholinergic neuron expression.Additionally,we used CoQ10 in intragastric administration to detect the profile of ChAT expression. Finally,we explored the neurophysiologic mechanisms underlying operative induced POCD.Methods Thirty-one healthy Wistar male rats aged 20 months weighting 500-550g were randomly divided into 4 groups:[A]control group(group C)received neither anesthesia nor surgery(n=7);[B]anesthesia group(group A)received intraperitoneal ketamine 25mg·Kg-1only(n=8);[C]surgery group(group S)received splenectomy under ketamine anesthesia(n=8);[D]Coenzyme Q10 group(group Co) were oral administrated CoQ10 30 mg·Kg-1·d-1during 21days(three weeks)before received the same treatment as group C(n=8).Morris Water Maze(MWM)was performed to assess the ability of spatial memory in the four groups on the 1st,3rd and 7th day after anesthesia or surgery.All rats were killed on the 7th day by perfused and fixed their brain using 4%paraformaldehyde.To measured the nerve cells on left hippocampus of every rat in HE staining sections;cholin acetyltransferase(ChAT)-positive neurons in hippocampus were detected by immunohistochemistry on the right.Results Compared with the other three groups, the latency to find the platform in MWM for group S was much longer on the 1st, 3rd and 7th day after surgery.There is no contrast in the four groups by HE staining sections.The cell numbers of ChAT-positive were obviously decreased in group s.Conclusion As splenectomy may contribute to an impairment of the hippocampus in age rats,stress reaction induced by the surgery resulted in deterioration of cognitive capacity.In addition,the results in the present study indicated that CoQ10 as an active participant,by neutralizing the deterioration,may play a crucial role in the functioning of cholinergic system in this animal model. |
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