The Expression Level In Serum Soluble Endoglin And Nitric Oxide Of Patients With Hypertensive Disorder Complicating Pregnancy

Objective:To investigate the role of sEng/NO in the pathogenesis of hypertensive disorder complicating pregnancy.To detect the sEng levels in serum of normal pregnant at different trimester.Methods:Choose 30 patients with hypertensive disorder complicating pregnancy(gestational hypertension 5 cases,mild preeclampsia 8 cases,severe preeclampsia 17 cases),normal pregnant women 50 cases(the first trimester 10 cases,the second trimester 10 cases,the third trimester 30 cases).Quantikine human sEng enzymelinked immunoassay(ELISA)kits were used to detect the serum level of sEng, the serum level of NO were detected by nitrite reductase kits.Results:1.The sEng levels and the NO levels in serum of normal pregnant at different trimester:(1)The sEng levels in serum of the first trimester group,the second trimester group and the third trimester group were 0.78±0.22 ng/ml,1.83±0.49 ng/ml,3.65±0.49 ng/ml,respectively.The sEng levels in the first trimester group lower than those in the second trimester group(P＜0.05), the second trimester group lower than the third trimester group(P＜0.05);(2)The NO levels in serum of the first trimester group,the second trimester group and the third trimester group were 34.39±7.52μmol/L,73.46±6.18μmol/L,78.17±8.09μmol/L,respectively.NO levels in the first trimester lower than the second trimester(P＜0.05),the second trimester lower than the third trimester(P＞0.05).2.The sEng levels and the NO levels in serum of the patients with hypertensive disorder complicating pregnancy:(1)The sEng levels in serum of the gestational hypertension group,the mild preeclamsia group and the sever preeclampsia group were 4.81±0.81 ng/ml,5.63±0.90 ng/ml,6.80±1.21 ng/ml,respectively,the gestational hypertension group were significantly higher than those in the normal third trimester group(P＜0.05),the mild preeclamsia group were higher than the gestational hypertension group,but had no significant different(P＞0.05).The sever preeclampsia group were significantly higher than the mild preeclamsia group and the gestational hypertension group (P＜0.05).(2)The NO levels in serum of the gestational hypertension group,the mild preeclamsia group and the sever preeclampsia group were 59.07±9.19μmol/L,54.73±11.77μmol/L,41.13±8.05μmol/L, respectively.The gestational hypertension were significantly lower than those in the normal third trimester group(P＜0.05).The severe preeclampsia group were significant lower than the mild preeclampsia (P＜0.05),there were no significant different between the mild preeclampsia group and the gestational hypertension group(P＞0.05).3.sEng levels were correlated positively with NO levels(r=-0.66, P＜0.01)in the patients with hypertensive disorder complicating pregnancy.Conclusions:1.The sEng levels increased and the NO levels decreased in patients with hypertensive disorder complicating pregnancy,the two factors correlates with disease severity.2.High sEng levels in serum may step down the NO levels then play a role in the pathophysiology of hypertensive disorder complicating pregnancy.