The Protective Effects Of Lipo PGE1 On Hepatic Injury Of Brain-dead Rats

ObjectiveIn this study,the rat models using the brain death of donors is established.Then the mechanism of the injury to livers from brain death of donors and its protection are well discussed to investigate the theoretical basis of using brain death of donors during Liver transplatation(LTx)in an effective way.MethodsTwenty-four SD rats were randomly allocated into 4 groups,6 rats in each group:control group(group C),brain-dead group(group B)and Lipo PGE1 protection group(group L1 and group L2).Brain-dead model were established in group B,L1 and L2.There is no inflation of Fogarty balloon in group C,the other operation was same with the group B.Lipo PGE1 at 20ng·Kg^-1·min^-1and 40ng·Kg^-1·min^-1was injected via the femoral vein in group L1 and group L2 immediately after the establishment of the brain-death model.Before increasing intracranial pressure(T1),the first time of confirmation of brain death(T2),after the first time of confirmation of brain death 6h(T3),blood from the femoral vein were abtained at the different time points.The serum levels of ALT and AST were cetected by biochemical analyzer.The serum levels of ET-1 were detected by radioimmunoassay.The serum levels of TNF-αwere detected by ELISA.Liver tissues were abtained for HE staining.All data were analyzed with statistic software SPSS 13.0.Results1.MAP:There is no significant difference among groupC,B,L1 andL2 before inflation of the Fogarty balloon(P＞0.05);in contrast to group C, the MAP in group B,L1and L2 was significantly higher during inflation of Fogarty balloon(P＜0.05),and was significantly lower in the state of brain-death(P＜0.05);there was no significantly diference among group B,L1 and L2 during inflation of Fogarty balloon and in the state of brain-death(P＞0.05).2.ALT,AST,TNF-α,ET-1:At the T1,T2 and T3 time points,there was significantly diference among the 4 group,(P＜0.05);at T3 time point,the levels of these parameters in group L1 and L2 was significantly lower than those in group B(P＜0.05);there was no significantly diference between group L1 and L2(P＞0.05).3.Morphological changes of hepatic tissues:in group C,hepatic tissues appearances were normal.In group B,obvious edema of partial cells was observed.Liver injury was significantly attenuated in group L₁ and group L₂.Conclusion1.The assay of eatablishing SD rats by increasing intracranial ressure in a modified,slow and intermittent way can effectively mimic the clinical brain death.2.Brain death causes damage to the donor liver of rats.So the hemodynamic change and inflammatory factor release may be the chief reasons.3.The release of TNF-α,ET-1 in the hepatocytes can be enhanced by the brain death,these serum inflammatory mediators may be involved in liver injury.4.Lipo PGE1 can attenuate the injury of the brain death donors.The protective mechanism of Lipo PGE1 may be decrease the release of the serum inflammatory mediators.