Relationship Between Dendritic Cell, Interleukin-23 And Ulcerative Colitis

Objective:Dendritic cell(DC)is a key antigen-presenting cell(APC)during cellular immune Responses,which can produce pro-inflammatory factor interleukin(IL)23.And IL-23 is a key cytokine which exerts strong inflammatory activities takes part in occurrence and development of ulcerative colitis(UC).The expression of S100+DC,CD83+DC and IL-23 in the mucosa of UC and irritable bowel syndrome(IBS)has been evaluated by immunohistochemistry(IHC).The aim of this study is to investigate the relationship between DC,IL-23 and UC to provide a theatrical basis for immunotherapy in treating UC.Methods:The lineage of 60 UC and 60 IBS colon tissue paraffin block was detected under colonoscope.The expression of S100+DC,CD83+DC and IL-23 was detected using immunohistochemical SP method.Then the distributions of the above three cells were reviewed,the infiltrated densities of them were calculated,and the relationships among them were analyzed.And their infiltrated densities were evaluated considering difference in stages,types,conditions and extent of UC.Results:1.The expression of S100+DC,CD83+DC and IL-23 can be detected in UC group and IBS group.In UC group,the positive-expression rates of S100+DC, CD83+DC and IL-23 were 86.7%,71.7%and 85.0%respectively;In IBS group, the positive-expression rates of the above three cells were 60.0%,66.7%and 70.0 %.There was no statistical difference between the positive expression rate of S100+DC and that of CD83+DC,while there remained significant differences between the two groups as far as IL-23 was concerned(P＜0.05).2.In UC group,the infiltrated densities of S100+DC,CD83+DC and IL-23 were 27.48±11.23,6.62±2.59 and 105.97±25.30;In IBS group,the infiltrated densities of the above three cells were 17.20±8.91,3.20±1.59 and 32.48±11.61.There was a statistical difference in the infiltrated densities of the above three cells between IBS group and UC group. 3.In IBS group and UC group,the infiltrated densities of S100+DC and CD83+DC have positive correlation with the infiltrated density that of IL-23.4.In UC group,the infiltrated densities of S100+DC,CD83+DC and IL-23 were 27.96±12.68,6.66±3.05 and 106.16±26.23 in mucosa or mucosa substratum;The infiltrated densities of the above three cells were 7.46±1.27,2.28±1.45 and 15.21±4.72 in muscular layer.There was a statistical difference in the infiltrated densities of the above three cells between mucosa or mucosa substratum and muscular layer.5.In UC group,the infiltrated densities of S100+DC,CD83+DC and IL-23 were 30.56±9.61,8.11±2.33 and 116.64±26.15 in initial type;The infiltrated densities of the above three cells were 22.83±8.07,4.38±2.08 and 89.38±22.79 in recur type. There was a statistical difference in the infiltrated densities of the above three cells between initial type and recur type.6.In UC group,the infiltrated densities of S100+DC,CD83+DC and IL-23 were 30.03±10.36,7.83±2.52 and 113.83±28.83 in acute episode stage;The infiltrated densities of the above three cells were 22.85±9.81,4.20±1.76,89.35±23.11 in chronic lag phase.There was a statistical difference in the infiltrated densities of the above three cells between acute episode stage and chronic lag phase.7.In UC group,the infiltrated densities of S100+DC,CD83+DC and IL-23 were 24.43±8.26,5.23±1.66,95.03±18.39 in mitis type;The infiltrated densities of the above three cells were 28.45±8.28,7.22±2.52,110.86±25.25 in moderate type;The infiltrated densities of the above three cells were 36.13±11.36,10.13±3.29, 133.38±25.32 in severe type.There was a statistical difference in the infiltrated densities of the above three cells between mitis type and severe type.8.In UC group,the infiltrated densities of S100+DC,CD83+DC and IL-23 were 28.11±10.35,6.59±2.64,105.81±26.73 in total colon type;The infiltrated densities of the above three cells were 26.94±9.83,6.64±2.61,103.14±24.48 in local colon type.There was no statistical difference in the infiltrated densities of the above three cells in different t extent of disease.Conclusions:1.In UC colon tissue,the infiltrated density and activity of DC increased,and the production of IL-23 increased,it offers reasonable theory to the immunity therapy based on IL-23 in UC.2.In UC colon tissue,the expression of S100+DC,CD83+DC and IL-23 was mainly in mucosa or mucosa substratum,which demonstrates DC and IL-23 expression in UC may be associated with altered immune,perhaps it offers a target to the therapy of UC.3.In UC colon tissue,the expression of S100+DC,CD83+DC and IL-23 was obviously increased with pathogenetic condition,so we can monitor the expression of S100+DC,CD83+DC and IL-23 in mucosa to learn the active degree and turnover prognosis of UC.