| Objective:Gastroenteric tumor is the most frequent tumor in our country. It has not special appearance in the earlier period , so most of the patients of Gastroenteric tumor in our country are in the advanced stage. The prognosis of this disease is not good , the recurrence after operation is high, the life span of the patients is short . At present Chem has been an important ingredient of combined therapy after the operationan ,an effective subsidiary device to extend the life of the malignant tumor patients. However the adverse reactions of Chem is not rarefactive. Bone marrow depression and gastrointestinal tract reaction are most common. In the process of Chem , due to the damage of Gastrointestinal mucous membrane from chemotherapeutics, the patients may show up nausea, emesia , diarrhea , severely membrana mucosa may be cellular necrosis ,ablate and the patients will show up hemafecia pantosomatous adverse effect.That will break off chems to influence clinical curative effect.Resently, there have been a lot of studies of the mechanism of action of chemotherapeutics causing impairment of stomach intestine barrierand how to lighten the adverse reaction of Chem, making a great advancement .But the studies in the comparison of impaired degree of gastrointestinal mucosa barrier between different chemotherapeutics is rarely reported .5-FU and ADM are two kinds of commonly chemotherapeutics in clinic.So we design the test to study and compare the difference of Gastrointestinal mucosa damage degree between 5-FU and ADM.,and help to choose more suitable Chem schema.for the patients in clinic.Methods: Sixty Wistar rats were randomly and averagely divided into three groups: A: control group, B: 5-Fu group, C:ADM group. After the rats were weighted, the rats in group A, B and C were given saline solution (50ml/kg) intragastric administrate respectively from day 1 to day 6. Group B were injected 5-Fu (100mg/kg)introperitoneally on the fourth day respectively to make the model of gut barrier injury.At.the.sametime,GroupCwere.injected .ADM(8mg/kg)introperitoneally and.group A received the same doses of saline solution injection introperitoneally. The content of endotoxin in peripheral blood was measured in all groups after rats were weighted and killed on the seventh day. The mesenteric lymph nodes (MLN) and livers were harvested for comparing bacterial translocation incidence among four groups. Ileum and colon tissues were taken for pathologic examination including comparing.mucosal thickness, villus height and width and morphology change of ileum and the weight of each group before and after the test.Statistical Analysis System 6.12 (SAS6.12) was used in the study. Analysis of variance and SNK-q test were used for quantitative.data.analysis,andFisher's.least-significant-differen ce test for qualitative data. P values that were less than 0.05 were considered statistical significance.Results:①Compared the weight of each group before and after the test:There was not significant difference among group A (346.20±12.72g), groupB (345.85±9.90g), groupC (349.45±12.97g) before test (P>0.05). After test, there was significant difference between group A (347.65±12.79g)and group B(317.75±9.63g) ,(P<0.05). group A and group C(333.9±13.85g), (P<0.05). The same result was obtained between group B and group C (P<0.05).②The content of endotoxin in peripheral blood of each group: significant difference is found between group A(0.037±0.012Eu/ml) and group B(0.091±0.013Eu/ml)(P<0.05), and the same beween group Aand group C (0.074±0.012Eu/ml)(P<0.05), group Band group C .The value of group C was significantly lower than that of group B (P<0.05).③The value of ileum villus height in each group: It was significantly higher in group A(364.48±12.05μm) than that in group B(275.06±11.91μm),group C(300.84±18.65μm) (P<0.05). That of group C were significantly higher than that of group B (P<0.05) . The value of was significantly different between group B and group C and group A (P<0.05).④The value of ileum villus width in each group : It was significantly wider in group A (173.96±21.34μm) than group B (112.48±15.85μm) andgroupC(137.96±11.90μm)(P<0.05). It was significantly wider in group C than group B (P<0.05). The value was significantly different between group B and group C (P<0.05) .⑤The value of ileum mucosal thickness in each group:It was significantly thicker in group A (542.67±27.88μm) than group B (420.10±16.82μm) and group C(455.04±12.30μm) (P<0.05). That of group C was significantly thicker than that of group B (P<0.05)..⑥The value of colon mucosal thickness in each group:It was significantly thicker in group A(421.10±31.24μm) than group B(262.03±31.30μm),group C(285.23±29.03μm) (P<0.05). That of group C was significantly thicker than that of group B (P<0.05).⑦The positive cases of bacterial translocation of MLN were found in group A(0/20),group B(17/20),group C(12/20) and the liver tissue were found in group A(0/20),group B(18/20),group C(14/20). The rate of bacterial translocation in group A was significantly lower than group C and group B (P<0.05). The rate of bacterial translocation in groupC was significantly lower than group B (P<0.05).Conclusions: 1.The rat's intestinal mucosa barrier is damaged during the intraperitoneal themothraphy of 5-Fu or ADM. 2. The damage effect of ADM to intestinal mucosa barrier is lower than that of 5-FU.
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